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PEI 介导体外 E1A 对肝癌细胞放射敏感性的影响。

The Effect of PEI-Mediated E1A on the Radiosensitivity of Hepatic Carcinoma Cells.

机构信息

Department of Radiology，Affiliated Hospital of Nanjing University of Chinese Medicine，Jiangsu Provincial Hospital of Traditional Chinese Medicine，Nanjing, Jiangsu Province ,China.

Department of Medical Imaging, Nanjing Vocational Health College, Nanjing, Jiangsu Province, China.

出版信息

Asian Pac J Cancer Prev. 2020 Apr 1;21(4):911-917. doi: 10.31557/APJCP.2020.21.4.911.

DOI:10.31557/APJCP.2020.21.4.911
PMID:32334450
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7445989/
Abstract

OBJECTIVE

The study was undertaken to investigate the effects of polyethyleneimine (PEI)-mediated adenovirus 5 early region 1A (E1A) on radiosensitivity of human hepatic carcinoma cell in vitro and to disclosure the underlying mechanism.

MATERIALS AND METHODS

Human hepatic carcinoma SMMC-7721 cell line was transfected with E1A gene using PEI vector. Untransfected cells (SMMC-7721 group), cells transfected with blank-vector (SMMC-7721-vect group), and cells transfected with E1A gene (SMMC-7721-E1A group) were treated with 6 MV X-ray irradiation at doses of 0, 1, 2, 4, 8 and Gy, respectively. Radiosensitivity was determined by MTT assay and quantified by calculating the cell survival rate. Cell-cycle distribution and apotosis rate were monitored by flow cytometry.

RESULTS

The survival rate of SMMC-7721-E1A was significantly lower than that of SMMC-7721 cell. Apoptosis rate of SMMC-7721-E1A group was significantly higher than that of SMMC-7721group (P<0.01).The ratio of S stage in cell cycle of SMMC-7721-E1A was significantly lower than that in SMMC-7721 cell. The ratio of G2/M stage in cell cycle of SMMC-7721-E1A was significantly higher than that in SMMC-7721 cell (P<0.01).

CONCLUSION

PEI could transfect E1A gene into hepatic carcinoma cells PEI-mediated E1A could effectively enhance radiosensitivity of hepatic carcinoma cells which may be related to its effects on apoptosis promoting leading to S phase suppression and G2/M phase arrest.
.

摘要

目的

研究聚乙烯亚胺（PEI）介导的腺病毒 5 早期区 1A（E1A）对人肝癌细胞体外放射敏感性的影响，并揭示其作用机制。

材料与方法

采用 PEI 载体转染人肝癌 SMMC-7721 细胞 E1A 基因。未转染细胞（SMMC-7721 组）、转染空白载体细胞（SMMC-7721-vect 组）和转染 E1A 基因细胞（SMMC-7721-E1A 组）分别接受 6MVX 射线照射，剂量分别为 0、1、2、4、8Gy。采用 MTT 法检测细胞放射敏感性，计算细胞存活率。采用流式细胞术检测细胞周期分布和凋亡率。

结果

SMMC-7721-E1A 细胞的存活率明显低于 SMMC-7721 细胞，SMMC-7721-E1A 组细胞的凋亡率明显高于 SMMC-7721 组（P<0.01）。SMMC-7721-E1A 细胞周期 S 期比例明显低于 SMMC-7721 细胞，G2/M 期比例明显高于 SMMC-7721 细胞（P<0.01）。

结论

PEI 可转染 E1A 基因进入肝癌细胞，PEI 介导的 E1A 可有效增强肝癌细胞的放射敏感性，可能与其促进凋亡、导致 S 期抑制和 G2/M 期阻滞有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6dd/7445989/628389749d8a/APJCP-21-0911-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6dd/7445989/8063a310b254/APJCP-21-0911-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6dd/7445989/702a6c8eb229/APJCP-21-0911-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6dd/7445989/9eb192eb6ebd/APJCP-21-0911-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6dd/7445989/1c2538d99371/APJCP-21-0911-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6dd/7445989/628389749d8a/APJCP-21-0911-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6dd/7445989/8063a310b254/APJCP-21-0911-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6dd/7445989/702a6c8eb229/APJCP-21-0911-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6dd/7445989/9eb192eb6ebd/APJCP-21-0911-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6dd/7445989/1c2538d99371/APJCP-21-0911-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6dd/7445989/628389749d8a/APJCP-21-0911-g005.jpg

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