Regenerative, Modular & Developmental Engineering Laboratory (REMODEL), Biomedical Sciences Building, National University of Ireland Galway (NUI Galway), Galway, Ireland; Science Foundation Ireland (SFI) Centre for Research in Medical Devices (CÚRAM), Biomedical Sciences Building, National University of Ireland Galway (NUI Galway), Galway, Ireland.
Merlin Park University Hospital, Galway, Ireland.
Methods Cell Biol. 2020;157:225-247. doi: 10.1016/bs.mcb.2019.11.003. Epub 2019 Nov 30.
Tendon injuries continuously rise, and regeneration is not only slow, but also limited due to the poor endogenous healing ability of the tendon tissue. Tissue grafts constitute the clinical gold standard treatment for severe injuries, but inherent limitations drive the field toward tissue engineering approaches to create suitable tissue constructs. Recapitulation of the native microenvironment represent a key challenge for the development of tendon tissue equivalents in vitro that can be further utilized as implantable devices. Methods to maintain cellular phenotype and to enhance extracellular matrix deposition for accelerated development of tissue-like modulus should be developed. Herein, we assessed the combining effect of surface topography and macromolecular crowding in human tenocyte culture. Our data demonstrated that bidirectionally aligned electrospun fibers induce physiological cell growth, while macromolecular crowding enhanced and accelerated tissue-specific extracellular matrix deposition. Collectively, these data advocate the use of multifactorial approaches for the accelerated development of functional tissue-like surrogates in vitro.
肌腱损伤不断增加，由于肌腱组织自身内源性愈合能力有限，其再生不仅缓慢，而且受限。组织移植物是严重损伤的临床金标准治疗方法，但固有局限性促使该领域转向组织工程方法来创建合适的组织构建体。在体外重现天然微环境是开发可进一步用作植入式装置的肌腱组织等效物的关键挑战。应开发保持细胞表型和增强细胞外基质沉积以加速组织样模量发展的方法。在此，我们评估了人肌腱细胞培养中表面形貌和大分子拥挤的联合效应。我们的数据表明，双向定向的静电纺丝纤维诱导生理细胞生长，而大分子拥挤则增强和加速了组织特异性细胞外基质的沉积。总的来说，这些数据支持使用多因素方法来加速体外功能性类似组织的发展。
