Bioengineering Division, Institute of Science, Hacettepe University, Ankara, Turkey.
Bioengineering Division, Institute of Science, Hacettepe University, Ankara, Turkey; Centre for Bioengineering, Hacettepe University, Ankara, Turkey.
Methods Cell Biol. 2020;157:49-79. doi: 10.1016/bs.mcb.2019.10.012. Epub 2019 Dec 10.
Supercritical carbon dioxide (scCO) is being used as an alternative approach to the traditional methods for the decellularization of tissues. This chapter describes the use of scCO for the decellularization of optic nerve, myocardium, and cornea tissues. The main goal of this method is to burst the cells with high-pressure, remove them from the tissues and to maintain the extracellular matrix structure of the native tissues. For this purpose, several scCO-assisted decellularization protocols were developed and optimized according to the requirements of these tissues. Efficiencies of the utilized decellularization protocols were determined via histological and morphological analysis. The decrease in the DNA content and the preserved glycosaminoglycan (GAG) amounts were also used as assessment parameters.
超临界二氧化碳（scCO2）正被用作替代传统组织脱细胞方法的一种手段。本章描述了使用 scCO2 对视神经、心肌和角膜组织进行脱细胞处理的方法。该方法的主要目的是通过高压使细胞破裂，将其从组织中去除，并保持天然组织的细胞外基质结构。为此，根据这些组织的要求，开发并优化了几种 scCO2 辅助脱细胞方案。通过组织学和形态学分析来确定所使用的脱细胞方案的效率。DNA 含量的减少和糖胺聚糖（GAG）含量的保留也被用作评估参数。
