Department of Otolaryngology, Affiliated ZhangJiaGang Hospital of Soochow University, Suzhou, 215600, China; Institute of Translational Medicine, Affiliated ZhangJiaGang Hospital of Soochow University, Suzhou, 215600, China.
Institute of Translational Medicine, Affiliated ZhangJiaGang Hospital of Soochow University, Suzhou, 215600, China.
Allergol Immunopathol (Madr). 2020 Jul-Aug;48(4):395-400. doi: 10.1016/j.aller.2019.11.005. Epub 2020 Apr 23.
Alpha-Tocopherol (α-TCP), one major form of vitamin E, has been known as a treatment for airway allergic inflammation. However, the role and mechanism of α-TCP in treating allergic rhinitis remains unclear.
In this study we examined the inhibitory function of α-TCP in a mouse model of allergic rhinitis.
Allergic phenotype was examined by hematoxylin and eosin staining. Total IgE, OVA-specific IgE, OVA-specific IgG1 and OVA-specific IgG2a levels were examined by ELISA. mRNA expression was measured by qPCR, protein levels were examined by Western Blot.
Histological analysis of the nasal membranes revealed that there was a significant reduction in inflammatory cells appearance in cross-sections in alpha-TCP treatment of Ovalbumin (OVA)-sensitized mice compared to OVA sensitized animals. In addition, eosinophils were significantly reduced in nasal mucosa of alpha-TCP treatment of OVA-sensitized mice compared to the OVA group. Lower total IgE, OVA-specific IgE, OVA-specific IgG1 and OVA-specific IgG2a levels were found in alpha-TCP treatment of OVA-sensitized mice compared to the OVA group. Furthermore, we found that the subepithelial distribution of tryptase positive mast cells was reduced in the alpha-TCP treatment of OVA-sensitized mice. More importantly, the PI3K-PKB pathway was suppressed by α-TCP in mast cells.
Our results demonstrated that α-TCP-mediated suppression of PI3K-PKB activity in mast cells is a potential mechanism of anti-allergic function of α-TCP.
α-生育酚（α-TCP）是维生素 E 的主要形式之一，已知可用于治疗气道过敏性炎症。然而，α-TCP 治疗变应性鼻炎的作用和机制尚不清楚。
本研究旨在探讨 α-TCP 在变应性鼻炎小鼠模型中的抑制作用。
采用苏木精-伊红染色观察过敏性表型。采用 ELISA 法检测总 IgE、OVA 特异性 IgE、OVA 特异性 IgG1 和 OVA 特异性 IgG2a 水平。采用 qPCR 检测 mRNA 表达，采用 Western blot 检测蛋白水平。
鼻黏膜组织学分析显示，与 OVA 致敏动物相比，α-TCP 治疗卵清蛋白（OVA）致敏小鼠的鼻腔切片中炎症细胞的出现明显减少。此外，与 OVA 组相比，α-TCP 治疗的 OVA 致敏小鼠鼻黏膜中的嗜酸性粒细胞明显减少。与 OVA 组相比，α-TCP 治疗的 OVA 致敏小鼠的总 IgE、OVA 特异性 IgE、OVA 特异性 IgG1 和 OVA 特异性 IgG2a 水平均降低。此外，我们发现，α-TCP 治疗的 OVA 致敏小鼠的亚上皮分布中，嗜碱性粒细胞中的类胰蛋白酶阳性细胞减少。更重要的是，α-TCP 抑制了肥大细胞中的 PI3K-PKB 通路。
我们的研究结果表明，α-TCP 通过抑制肥大细胞中的 PI3K-PKB 活性，介导其抗变应性作用的潜在机制。
