Samsel J, Petit A, Weniger J P
Laboratoire de Zoologie et d'Embryologie expérimentale de l'Université Louis Pasteur, Strasbourg, France.
Cell Differ Dev. 1988 Dec;25(3):223-8. doi: 10.1016/0922-3371(88)90119-0.
Gonads dissected from 17-day-old normal chick embryos, as well as testes feminized by oestrogen treatment, were labelled in vitro with [35S]methionine and [14C]leucine. The patterns of cytosolic protein synthesis by the gonads were analysed using two-dimensional (2-D) polyacrylamide gel electrophoresis. In the feminized testis, two female-specific proteins appeared, the rate of synthesis of four testis-specific proteins was decreased, and quantitative variations of other synthesized proteins in relation to sex were detected. This altered protein synthesis indicates that the protein synthesis of the testis feminized by oestrogen treatment has in part become similar to that of a normal ovary. The synthesis of female-specific proteins in the feminized testis raises the question of their role in normal ovarian differentiation.
从17日龄正常鸡胚中分离出的性腺，以及经雌激素处理而雌性化的睾丸，在体外用[35S]甲硫氨酸和[14C]亮氨酸进行标记。使用二维（2-D）聚丙烯酰胺凝胶电泳分析性腺胞质蛋白的合成模式。在雌性化睾丸中，出现了两种雌性特异性蛋白，四种睾丸特异性蛋白的合成速率降低，并且检测到其他合成蛋白相对于性别的定量变化。这种改变的蛋白合成表明，经雌激素处理雌性化的睾丸的蛋白合成部分已变得类似于正常卵巢的蛋白合成。雌性化睾丸中雌性特异性蛋白的合成提出了它们在正常卵巢分化中的作用问题。
