Ferdousy Raihana Nasrin, Kereilwe Onalenna, Kadokawa Hiroya
Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi-shi, Yamaguchi-ken 1677-1, Japan.
Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi-shi, Yamaguchi-ken 1677-1, Japan; and Corresponding author. Email address:
Reprod Fertil Dev. 2020 May;32(8):738-747. doi: 10.1071/RD19121.
Anti-Müllerian hormone (AMH) is a glycoprotein produced by granulosa cells of preantral and small antral follicles that has multiple important roles in the ovaries. Recent studies have revealed extragonadal AMH regulation of gonadotrophin secretion from bovine gonadotrophs. In this study we investigated whether the primary receptor for AMH, AMH receptor type 2 (AMHR2), is expressed in bovine oviducts and endometria. Reverse transcription-polymerase chain reaction detected expression of AMHR2 mRNA in oviductal and endometrial specimens. Western blotting and immunohistochemistry were performed to analyse AMHR2 protein expression using anti-bovine AMHR2 antibody. Immunohistochemistry revealed robust AMHR2 expression in the tunica mucosa of the ampulla and isthmus, as well as in the glandular and luminal epithelium of the endometrium. AMHR2 mRNA (measured by real-time polymerase chain reaction) and AMHR2 protein expression in these layers did not significantly differ among oestrous phases in adult Wagyu cows (P>0.1). In addition, AMHR2 mRNA and protein expression in these layers did not differ among old Holsteins (mean (±s.e.m.) age 91.9±6.4 months) and young (26.6±0.8 months) and old (98.8±10.2 months) Wagyu cows. Therefore, AMHR2 is expressed in bovine oviducts and endometria.
抗苗勒管激素（AMH）是由窦前卵泡和小窦卵泡的颗粒细胞产生的一种糖蛋白，在卵巢中具有多种重要作用。最近的研究揭示了性腺外AMH对牛促性腺激素细胞分泌促性腺激素的调节作用。在本研究中，我们调查了AMH的主要受体——2型AMH受体（AMHR2）是否在牛输卵管和子宫内膜中表达。逆转录-聚合酶链反应检测到输卵管和子宫内膜标本中AMHR2 mRNA的表达。使用抗牛AMHR2抗体进行蛋白质免疫印迹和免疫组织化学分析AMHR2蛋白的表达。免疫组织化学显示，壶腹部和峡部的黏膜层以及子宫内膜的腺上皮和腔上皮中均有强烈的AMHR2表达。成年和牛母牛发情期各层中的AMHR2 mRNA（通过实时聚合酶链反应测量）和AMHR2蛋白表达无显著差异（P>0.1）。此外，在老龄荷斯坦奶牛（平均（±标准误）年龄91.9±6.4个月）以及年轻（26.6±0.8个月）和老龄（98.8±10.2个月）和牛母牛的这些层中AMHR2 mRNA和蛋白表达也无差异。因此，AMHR2在牛输卵管和子宫内膜中表达。
