Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.
Anim Sci J. 2020 Jan-Dec;91(1):e13456. doi: 10.1111/asj.13456.
In this study, we investigated whether bovine oviducts and endometria produce anti-Müllerian hormone (AMH) (for paracrine and autocrine signaling). Reverse transcription-polymerase chain reaction and western blotting detected AMH expression in oviductal and endometrial specimens. Immunohistochemistry revealed robust AMH expression in the ampulla and isthmus epithelia, and the glandular and luminal endometrial epithelia (caruncular endometria). AMH mRNA (measured by real-time PCR) and protein expression in these layers did not significantly differ among estrous phases in adult Japanese Black (JB) heifers (p > .1). Furthermore, the expression in these layers also did not differ among Holstein cows (93.8 ± 5.8 months old), JB heifers (25.5 ± 0.4 months old), and JB cows (97.9 ± 7.9 months old). We also compared AMH concentrations in the oviduct and uterine horn fluids among the three groups (measured by immunoassays). Interestingly, the AMH concentration in the oviduct fluid, but not in the uterine horn fluid, of Holstein cows was lower than those in JB heifers and cows (p < .05). Therefore, bovine oviducts and endometria express AMH and likely secrete it into the oviduct and uterine fluids.
在这项研究中，我们调查了牛输卵管和子宫内膜是否产生抗缪勒管激素 (AMH)（用于旁分泌和自分泌信号）。逆转录聚合酶链反应和 Western blot 检测到输卵管和子宫内膜标本中的 AMH 表达。免疫组织化学显示 AMH 在输卵管壶腹部和峡部上皮以及腺体和腔内膜上皮（肉阜内膜）中表达强烈。在成年日本黑（JB）小母牛（p>.1）的发情周期中，这些层中的 AMH mRNA（通过实时 PCR 测量）和蛋白表达没有显著差异。此外，这些层中的表达也没有在荷斯坦奶牛（93.8±5.8 个月）、JB 小母牛（25.5±0.4 个月）和 JB 奶牛（97.9±7.9 个月）之间存在差异。我们还比较了三组牛的输卵管和子宫角液中的 AMH 浓度（通过免疫测定测量）。有趣的是，荷斯坦奶牛的输卵管液中的 AMH 浓度（而不是子宫角液中的）低于 JB 小母牛和奶牛（p<.05）。因此，牛的输卵管和子宫内膜表达 AMH 并可能将其分泌到输卵管和子宫液中。
