Meiniel R, Duchier N, Meiniel A
Laboratoire de Biologie animale, Université Blaise Pascal, Clermont-Ferrand, France.
Cell Tissue Res. 1988;254(3):611-5. doi: 10.1007/BF00226511.
To obtain specific immunological probes for investigation of the cellular and molecular aspects of the subcommissural organ (SCO), we produced monoclonal antibodies directed against extracts from the bovine SCO. An hybridoma cell line (C1A8B8) was isolated by screening the culture media by means of the immunofluorescence method. This clone produces an IgG1 that recognizes the ventricular secretory material of the SCO including Reissner's fiber. A competition test using C1B8A8 immunoglobulin and lectins (concanavalin A and wheat-germ agglutinin) was applied to demonstrate that both the immature and mature forms of the glycoprotein were recognized. This antibody will offer a good tool for immunocytochemical localization and immunoaffinity purification of the antigen and for isolation of cDNA clones encoding it.
为了获得用于研究连合下器官（SCO）细胞和分子层面的特异性免疫探针，我们制备了针对牛SCO提取物的单克隆抗体。通过免疫荧光法筛选培养基，分离出了一个杂交瘤细胞系（C1A8B8）。该克隆产生一种IgG1，可识别SCO的脑室分泌物质，包括赖氏纤维。使用C1B8A8免疫球蛋白和凝集素（伴刀豆球蛋白A和麦胚凝集素）进行竞争试验，以证明糖蛋白的未成熟和成熟形式均能被识别。这种抗体将为抗原的免疫细胞化学定位和免疫亲和纯化以及编码该抗原的cDNA克隆的分离提供一个良好的工具。
