Spitz M, Spitz L, Thorpe R, Eugui E
J Immunol Methods. 1984 May 11;70(1):39-43. doi: 10.1016/0022-1759(84)90387-9.
A novel immunization procedure for eliciting monoclonal antibodies ( McAbs ) is described. With intrasplenic inoculation only small amounts of immunogen are required. As little as 20 micrograms of protein antigen or 2.5 X 10(5) cells have been found sufficient to immunize mice or rat spleen cells for the production of specific McAbs . A high proportion of hybridomas secreting McAbs against cell surface antigens and soluble proteins has been obtained with this immunization procedure. The system could facilitate McAb production in many instances in which only small quantities of immunogen are available.
描述了一种用于产生单克隆抗体（McAbs）的新型免疫程序。通过脾内接种，仅需少量免疫原。已发现低至20微克蛋白质抗原或2.5×10⁵个细胞就足以免疫小鼠或大鼠脾细胞以产生特异性单克隆抗体。通过这种免疫程序已获得了高比例的分泌针对细胞表面抗原和可溶性蛋白质的单克隆抗体的杂交瘤。在许多仅可获得少量免疫原的情况下，该系统可促进单克隆抗体的产生。
