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R. F. 埃利奥特1968年关于鲤鱼(布洛赫,1782年)鱼卵感染及其用草药提取物进行控制的研究

R. F. Elliott 1968 infection in Prussian carp (Bloch, 1782) eggs and its control with herb extracts.

作者信息

Cao H, Ou R, Li G, Yang X, Zheng W, Lu L

机构信息

Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture of P. R. China, Shanghai Engineering Research Center of Aquaculture, National Pathogen Collection Center for Aquatic Animals Shanghai University Knowledge Service Platform, Shanghai Ocean University Aquatic Animal Breeding Center (ZF1206), Shanghai Ocean University Shanghai China.

Sihong Bureau of Aquatic Products Sihong China.

出版信息

J Appl Ichthyol. 2014 Feb;30(1):145-150. doi: 10.1111/jai.12316. Epub 2013 Aug 26.

DOI:10.1111/jai.12316
PMID:32336844
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7166355/
Abstract

In order to control saprolegniosis in Prussian carp ( (Bloch, 1782) eggs, it is important to screen herb extracts as potential anti- drugs in Prussian carp hatcheries. For this purpose, an oomycete water mould (strain SC) isolated from Prussian carp [ (Bloch, 1782)] eggs suffering from saprolegniosis was characterised morphologically as well as from ITS rDNA sequence data. Initially identified as a sp. based on its morphological features, the constructed phylogenetic tree using the neighbour joining method further indicated that the SC strain was closely related to R. F. Elliott 1968 strain VI05733 (GenBank accession no. HE798564), and which could form biofilm communities as virulence factors. In addition, aqueous extracts from forty Chinese herbs were screened as possible anti- agents. Among them, a 1 g ml extract from was the most efficacious anti- agent, indicated by the minimum inhibitory concentration that was as low as 256 mg L. Relative survival of 73 and 88% was obtained against the SC strain in fish eggs at concentrations of 256 and 1280 mg L, respectively. This is the first known report of R. F. Elliott 1968 infection in (Bloch, 1782) eggs involving the screening of extracts as potential anti- agents.

摘要

为了控制鲤鱼((布洛赫,1782年))鱼卵的水霉病,在鲤鱼孵化场筛选作为潜在抗真菌药物的草药提取物很重要。出于这个目的,从患有水霉病的鲤鱼[(布洛赫,1782年)]鱼卵中分离出的一种卵菌类水霉菌(菌株SC),从形态学以及ITS rDNA序列数据方面进行了特征描述。最初根据其形态特征鉴定为一种 菌,使用邻接法构建的系统发育树进一步表明,SC菌株与R. F. 埃利奥特1968年的VI05733菌株(GenBank登录号HE798564)密切相关,并且它可以形成生物膜群落作为毒力因子。此外,对40种中草药的水提取物进行了筛选,以寻找可能的抗真菌剂。其中,来自 的1 g/ml提取物是最有效的抗真菌剂,其最低抑菌浓度低至256 mg/L。在鱼卵中,浓度为256和1280 mg/L时,对SC菌株的相对存活率分别为73%和88%。这是关于R. F. 埃利奥特1968年在鲤鱼((布洛赫,1782年))鱼卵中的感染涉及筛选提取物作为潜在抗真菌剂的首次已知报道。

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