Su Shenghui, Chen Weidong, Zheng Minghui, Lu Guozan, Tang Wei, Huang Haihong, Qu Dongbin
Division of Spine Surgery, Department of Orthopaedics, Nanfang Hospital, Southern Medical University, 510515 Guangzhou, China.
Department of Orthopaedic Surgery, Zengcheng Branch of Nanfang Hospital, Southern Medical University, 511338 Guangzhou, China.
ACS Omega. 2022 Mar 1;7(10):8391-8402. doi: 10.1021/acsomega.1c05908. eCollection 2022 Mar 15.
To improve osseointegration caused by the stress-shielding effect and the inert nature of titanium-based alloys, in this work, we successfully constructed a strontium calcium phosphate (Sr-CaP) coating on three-dimensional (3D)-printed Ti6Al4V scaffolds to address this issue. The energy-dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD) results indicated that the coatings with and without Sr doping mainly consisted of CaHPO. The bonding strength of Sr doping coating met the required ISO 13 779-4-2018 standard (≥15 MPa). The results suggested that the Sr-CaP-modified Ti6Al4V scaffolds were found to effectively promote mice bone-marrow stem cell (mBMSC) adhesion, spreading, and osteogenesis. The experiments also showed that the Sr-CaP-modified Ti6Al4V scaffolds could significantly improve bone regeneration and osseointegration. More importantly, Sr-doped CaP-coated Ti6Al4V scaffolds were found to accelerate bone healing in comparison to CaP-coated Ti6Al4V scaffolds. The Sr-CaP-modified Ti6Al4V scaffolds are considered a promising strategy to develop bioactive surfaces for enhancing the osseointegration between the implant and bone tissue.
为改善因应力屏蔽效应和钛基合金的惰性而导致的骨整合，在本研究中，我们成功地在三维（3D）打印的Ti6Al4V支架上构建了磷酸锶钙（Sr-CaP）涂层以解决该问题。能量色散X射线光谱（EDS）和X射线衍射（XRD）结果表明，含Sr掺杂和不含Sr掺杂的涂层主要由磷酸氢钙组成。Sr掺杂涂层的结合强度符合ISO 13 779-4-2018标准要求（≥15 MPa）。结果表明，Sr-CaP修饰的Ti6Al4V支架能有效促进小鼠骨髓干细胞（mBMSC）的黏附、铺展和成骨。实验还表明，Sr-CaP修饰的Ti6Al4V支架能显著改善骨再生和骨整合。更重要的是，与CaP涂层的Ti6Al4V支架相比，发现Sr掺杂的CaP涂层的Ti6Al4V支架能加速骨愈合。Sr-CaP修饰的Ti6Al4V支架被认为是一种有前景的策略，可用于开发生物活性表面以增强植入物与骨组织之间的骨整合。
