Fu Hanjiang, Xue Yong, Su Fei, Ding Kexin, Wang Yuan, Yu Haiyue, Zhu Jie, Li Qing, Ge Changhui, Zheng Xiaofei
Department of Experimental Hematology and Biochemistry, Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing, China.
College of Life Science and Technology, Office of Academic Affairs, Dalian University, Dalian, Liaoning, China.
Dose Response. 2020 Apr 20;18(2):1559325820920141. doi: 10.1177/1559325820920141. eCollection 2020 Apr-Jun.
During large-scale acute radiation exposure, rapidly distinguishing exposed individuals from nonexposed individuals is necessary. Identifying those exposed to high and potentially lethal radiation doses, and in need of immediate treatment, is especially important. To address this and find plasma biomarkers to assess ionizing radiation-induced mortality in the early stages, mice were administered a whole-body lethal dose of γ radiation, and radiation-induced damage was evaluated. Multiple blood biomarkers were screened using an antibody array, followed by validation using enzyme-linked immunoassay. The results revealed that irradiation (IR)-induced mortality in mice and caused body weight and blood platelet losses in deceased mice compared to surviving mice. The levels of certain proteins differed after IR between these 2 groups. Specific proteins in preirradiated mice were also found to potentiate radiosensitivity. Plasma levels of interleukin (IL)-22, urokinase, resistin, and IL-6 were associated with radiation-induced mortality in irradiated mice and may be useful as potential mortality predictors. Our results suggest that estimating the levels of certain plasma proteins is a promising alternative to conventional cytogenetic biodosimetry to accurately identify individuals exposed to high radiation doses and those at risk of death due to exposure. This strategy would facilitate the rapid triage of individuals requiring immediate and intensive medical treatment.
在大规模急性辐射暴露期间，迅速区分受辐射个体和未受辐射个体很有必要。识别那些受到高剂量且可能致命的辐射、需要立即治疗的个体尤为重要。为解决这一问题并寻找血浆生物标志物以在早期评估电离辐射导致的死亡率，给小鼠全身给予致死剂量的γ辐射，并对辐射诱导的损伤进行评估。使用抗体阵列筛选多种血液生物标志物，随后通过酶联免疫吸附测定进行验证。结果显示，与存活小鼠相比，辐射（IR）导致小鼠死亡，并使死亡小鼠出现体重减轻和血小板减少。这两组小鼠在IR后某些蛋白质的水平有所不同。还发现照射前小鼠中的特定蛋白质会增强放射敏感性。白细胞介素（IL）-22、尿激酶、抵抗素和IL-6的血浆水平与受辐照小鼠的辐射诱导死亡率相关，可能作为潜在的死亡率预测指标。我们的结果表明，估计某些血浆蛋白质的水平是传统细胞遗传学生物剂量测定法的一种有前景的替代方法，可准确识别暴露于高辐射剂量的个体以及因辐射而有死亡风险的个体。该策略将有助于对需要立即和强化医疗治疗的个体进行快速分类。
