Biscans B, Alinat P, Bertrand J, Sanchez V
Laboratoire de Génie Chimique, UA, Toulouse, France.
Electrophoresis. 1988 Feb;9(2):84-9. doi: 10.1002/elps.1150090205.
Continuous flow zone electrophoresis is an efficient method for the non-destructive separation of biological materials in a flowing film of buffer solution in which the constituents of a sample are separated according to their electrophoretic mobilities, under the influence of an electric field. This paper deals with modeling of flow structure of the buffer solution taking into account the effect of electroosmosis, Joule heating and thermal free convection and the forced convection of axial flow. Modeling of diffusion is examined in a second part. The modeling equations and the corresponding boundary conditions are solved by finite difference methods. The various parameters affecting the quality of the fractionation are analyzed.
连续流动区带电泳是一种在缓冲溶液流动膜中对生物材料进行无损分离的有效方法，在电场影响下，样品成分根据其电泳迁移率进行分离。本文考虑电渗、焦耳热、热自由对流和轴向流的强制对流的影响，对缓冲溶液的流动结构进行建模。第二部分研究扩散建模。通过有限差分法求解建模方程和相应的边界条件。分析了影响分级质量的各种参数。
