miR-99b 通过靶向 IGF1R 调节脑缺血神经元损伤。

MiR-99b regulates cerebral ischemia neuronal injury through targeting IGF1R.

机构信息

Department of Neurology, Affiliated Hospital of Jining Medical University, YanZhou Branch, Jining, China.

Disinfection Supply Center, Qingdao Municipal Hospital, Qingdao, China.

出版信息

Panminerva Med. 2023 Mar;65(1):30-36. doi: 10.23736/S0031-0808.20.03920-8. Epub 2020 Apr 28.

DOI:10.23736/S0031-0808.20.03920-8

PMID:32343508

Abstract

BACKGROUND

Recently, microRNA-99b (miR-99b) shows diverse functions in different human disease. However, further studies about the potential effect of miR-99b in cerebral ischemia injury still need to be done.

METHODS

The expressions of miR-99b and IGF1R were detected via RT-qPCR assay. Western blot assay was applied to measure the protein expression of Caspase-3, Bax and Bcl-2. MTT assay was used to observe cell viability of SH-SY5Y cells. The association of miR-99b and IGF1R was testified by dual luciferase assay. And human SH-SY5Y cells were treated with the oxygen-glucose deprivation/reperfusion (OGD/R) to mimic CIR injury.

RESULTS

The expression of miR-99b was increased in the OGD/R model. And upregulation of miR-99b promoted cell viability and inhibited apoptosis induced by OGD/R. Moreover, IGF1R was confirmed as a direct target gene of miR-99b. The expression of IGF1R was obviously decreased under OGD/R conditions.

CONCLUSIONS

MiR-99b promoted the viability and suppressed apoptosis of SH-SY5Y cells under OGD/R conditions through targeting IGF1R.

摘要

背景

最近，miR-99b（miR-99b）在不同的人类疾病中表现出多种功能。然而，仍需要进一步研究 miR-99b 在脑缺血损伤中的潜在作用。

方法

通过 RT-qPCR 检测 miR-99b 和 IGF1R 的表达。Western blot 检测 Caspase-3、Bax 和 Bcl-2 的蛋白表达。MTT 法观察 SH-SY5Y 细胞活力。双荧光素酶实验验证 miR-99b 和 IGF1R 的关联。用氧葡萄糖剥夺/再灌注（OGD/R）处理人 SH-SY5Y 细胞模拟 CIR 损伤。