Department of Chemical Engineering, Institute of Chemical Technology, Matunga (E), Mumbai, 400019, India.
Department of Chemical Engineering, Institute of Chemical Technology, Matunga (E), Mumbai, 400019, India.
J Biotechnol. 2020 Jun 20;317:27-33. doi: 10.1016/j.jbiotec.2020.04.010. Epub 2020 Apr 25.
In conventional preparation of enzyme embedded organic-inorganic hybrid flower-like nanostructures, usually it requires three days which is time-consuming and limits their widespread applications. In this context, alpha-amylase hybrid bio-microflowers were prepared by simple, efficient and rapid method in the presence of chitosan via inotropic gelation and biomineralization approach. The hybrid bio-microflowers was synthesized within 6 h with ∼140 % enhanced catalytic activity. The prepared hybrid bio-microflowers were characterized by FT-IR, SEM, and PXRD. The hybrid bio-microflowers exhibited higher rate of reaction (V) and outstanding thermo-stability (in the temperature range 55-75 °C). Further, hybrid bio-microflowers showed magnificent reusability (upto eight cycles) and long-term storage stability (for about 30 days). In the end, in starch hydrolytic study, immobilized alpha-amylase exhibited higher hydrolytic potential towards corn, wheat and potato starch as compared to free form. This designed hybrid bio-microflowers can be employed as an engineered biocatalyst in industrial applications.
在传统的酶嵌入有机-无机杂化花状纳米结构的制备中，通常需要三天的时间，这既耗时又限制了它们的广泛应用。在这种情况下，通过向性凝胶化和生物矿化方法，在壳聚糖存在的条件下，通过简单、高效和快速的方法制备了α-淀粉酶杂化生物微花。在 6 小时内合成了杂化生物微花，催化活性提高了约 140%。通过傅里叶变换红外光谱（FT-IR）、扫描电子显微镜（SEM）和粉末 X 射线衍射（PXRD）对杂化生物微花进行了表征。杂化生物微花表现出更高的反应速率（V）和出色的热稳定性（在 55-75°C 的温度范围内）。此外，杂化生物微花具有出色的可重复使用性（可达 8 次循环）和长期储存稳定性（约 30 天）。最后，在淀粉水解研究中，固定化α-淀粉酶对玉米、小麦和马铃薯淀粉的水解潜力明显高于游离形式。这种设计的杂化生物微花可作为工程生物催化剂在工业应用中使用。
