一种具有增强活性的快速自组装混合生物微花的α-淀粉酶。
A rapid self-assembled hybrid bio-microflowers of alpha-amylase with enhanced activity.
机构信息
Department of Chemical Engineering, Institute of Chemical Technology, Matunga (E), Mumbai, 400019, India.
Department of Chemical Engineering, Institute of Chemical Technology, Matunga (E), Mumbai, 400019, India.
出版信息
J Biotechnol. 2020 Jun 20;317:27-33. doi: 10.1016/j.jbiotec.2020.04.010. Epub 2020 Apr 25.
In conventional preparation of enzyme embedded organic-inorganic hybrid flower-like nanostructures, usually it requires three days which is time-consuming and limits their widespread applications. In this context, alpha-amylase hybrid bio-microflowers were prepared by simple, efficient and rapid method in the presence of chitosan via inotropic gelation and biomineralization approach. The hybrid bio-microflowers was synthesized within 6 h with ∼140 % enhanced catalytic activity. The prepared hybrid bio-microflowers were characterized by FT-IR, SEM, and PXRD. The hybrid bio-microflowers exhibited higher rate of reaction (V) and outstanding thermo-stability (in the temperature range 55-75 °C). Further, hybrid bio-microflowers showed magnificent reusability (upto eight cycles) and long-term storage stability (for about 30 days). In the end, in starch hydrolytic study, immobilized alpha-amylase exhibited higher hydrolytic potential towards corn, wheat and potato starch as compared to free form. This designed hybrid bio-microflowers can be employed as an engineered biocatalyst in industrial applications.
在传统的酶嵌入有机-无机杂化花状纳米结构的制备中，通常需要三天的时间，这既耗时又限制了它们的广泛应用。在这种情况下，通过向性凝胶化和生物矿化方法，在壳聚糖存在的条件下，通过简单、高效和快速的方法制备了α-淀粉酶杂化生物微花。在 6 小时内合成了杂化生物微花，催化活性提高了约 140%。通过傅里叶变换红外光谱（FT-IR）、扫描电子显微镜（SEM）和粉末 X 射线衍射（PXRD）对杂化生物微花进行了表征。杂化生物微花表现出更高的反应速率（V）和出色的热稳定性（在 55-75°C 的温度范围内）。此外，杂化生物微花具有出色的可重复使用性（可达 8 次循环）和长期储存稳定性（约 30 天）。最后，在淀粉水解研究中，固定化α-淀粉酶对玉米、小麦和马铃薯淀粉的水解潜力明显高于游离形式。这种设计的杂化生物微花可作为工程生物催化剂在工业应用中使用。
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