College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, China; College of Life Science and Technology, Southwest University for Nationalities, Chengdu, 610041, China.
College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, China.
Ecotoxicol Environ Saf. 2020 Jul 15;198:110619. doi: 10.1016/j.ecoenv.2020.110619. Epub 2020 Apr 25.
Ammonia (NH), an environmental pollutant with a pungent odor, is not only an important volatile in fertilizer production and ranching, but also main basic component of haze. In present study, we found that ultrastructural changes and 3167 differentially expressed proteins (DEPs) using proteomics analysis in the thymuses of chickens exposed to NH on day 42. Obtained DEPs were enriched using GO and KEGG; and 66 DEPs took part in immune function, metabolic process, and apoptosis in the thymuses of chickens treated with NH. 9 genes of DEPs were validated using qRT-PCR, and mRNA expression of 2 immune-related genes (CTSG and NFATC2), 3 metabolic process-related genes (APOA1, GOT1, and GOLGA3), and 4 apoptosis-related genes (PIK3CD, CTSS, CAMP, and NSD2) were consistent with DEPs in chicken thymuses. Our results indicated that excess NH led to immunosuppression, metabolic disorder, and apoptosis in chicken thymuses. Present study gives a novel insight into the mechanism of NH toxicity and demonstrated that immune response, metabolism process, and apoptosis were important in the mechanism of NH toxicity of chicken exposure to high concentration of NH.
氨（NH）是一种具有刺激性气味的环境污染物，不仅是肥料生产和牧场的重要挥发性物质，也是霾的主要基本成分。在本研究中，我们发现暴露于 NH42 天的鸡的胸腺中存在超微结构变化和 3167 个差异表达蛋白（DEPs）。使用蛋白质组学分析获得的 DEPs 进行了 GO 和 KEGG 富集；并且 66 个 DEPs 参与了 NH 处理的鸡的胸腺中的免疫功能、代谢过程和细胞凋亡。使用 qRT-PCR 验证了 9 个 DEP 基因，并且 2 个免疫相关基因（CTSG 和 NFATC2）、3 个代谢过程相关基因（APOA1、GOT1 和 GOLGA3）和 4 个凋亡相关基因（PIK3CD、CTSS、CAMP 和 NSD2）的 mRNA 表达与鸡胸腺中的 DEPs 一致。我们的结果表明，过量的 NH 导致鸡胸腺中的免疫抑制、代谢紊乱和细胞凋亡。本研究为 NH 毒性的机制提供了新的见解，并表明免疫反应、代谢过程和细胞凋亡在鸡暴露于高浓度 NH 时的 NH 毒性机制中很重要。
