School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Academy of Contemporary Food Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou 510006, China; Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods, & Guangdong Province Engineering Laboratory for Intelligent Cold Chain Logistics Equipment for Agricultural Products, Guangzhou Higher Education Mega Center, Guangzhou 510006, China.
School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Academy of Contemporary Food Engineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou 510006, China; Engineering and Technological Research Centre of Guangdong Province on Intelligent Sensing and Process Control of Cold Chain Foods, & Guangdong Province Engineering Laboratory for Intelligent Cold Chain Logistics Equipment for Agricultural Products, Guangzhou Higher Education Mega Center, Guangzhou 510006, China; Food Refrigeration and Computerized Food Technology (FRCFT), Agriculture and Food Science Centre, University College Dublin, National University of Ireland, Belfield, Dublin 4, Ireland.
Food Chem. 2020 Sep 15;324:126832. doi: 10.1016/j.foodchem.2020.126832. Epub 2020 Apr 21.
Aflatoxin B1 (AFB1) as the most toxic mycotoxin in contaminated food can greatly threaten human health, and sensitive and selective detection of AFB1 is thus highly desired. An ultrasensitive surface-enhanced Raman spectroscopy (SERS) aptasensor was developed for AFB1 detection in peanut oil samples. SH-cDNA modified FeO@Au nanoflowers acted as capture probes, SH-Apt modified Au@Ag nanospheres and commercial Cy3-Apt were used as reporter probes. Strong SERS signals of reporter probes were produced due to the recognition of AFB1 aptamer and its complementary strand (SH-cDNA). With the preferred binding of AFB1 aptamer to AFB1, reporter probes were released from capture probes, causing a linear decrease in SERS intensity. Therefore an ultralow detection limit of 0.40 pg·mL in a wide linear range of 0.0001-100 ng·mL was obtained and the sensibility of this SERS aptasensor was higher than that of the Cy3-Apt based SERS aptasensor. In addition, an excellent selectivity in interfering toxins and satisfactory recoveries of 96.6-115% in peanut oil samples were obtained, proving this aptasensor is a promising analytical tool in AFB1 detection.
黄曲霉毒素 B1（AFB1）作为污染食品中最具毒性的霉菌毒素，极大地威胁着人类健康，因此，人们非常希望能够对其进行敏感和选择性检测。本研究开发了一种用于花生油样品中 AFB1 检测的超灵敏表面增强拉曼光谱（SERS）适体传感器。SH-cDNA 修饰的 FeO@Au 纳米花作为捕获探针，SH-Apt 修饰的 Au@Ag 纳米球和商用 Cy3-Apt 作为报告探针。由于 AFB1 适体及其互补链（SH-cDNA）的识别，报告探针产生了强的 SERS 信号。由于 AFB1 适体与 AFB1 的优先结合，报告探针从捕获探针上释放出来，导致 SERS 强度线性下降。因此，在 0.0001-100ng·mL 的较宽线性范围内，获得了 0.40pg·mL 的超低检测限，并且该 SERS 适体传感器的灵敏度高于基于 Cy3-Apt 的 SERS 适体传感器。此外，该适体传感器在干扰毒素方面具有优异的选择性，在花生油样品中的回收率为 96.6-115%，证明该适体传感器是一种有前途的 AFB1 检测分析工具。
