Mahmoud Manal A M, Megahed Gaber, Yousef Mohamed S, Ali Fatma Abo Zakaib, Zaki Rania S, Abdelhafeez Hanan H
Department of Animal Hygiene, Faculty of Veterinary Medicine, Assiut University, 71526 Assiut, Egypt.
Department of Theriogenology, Faculty of Veterinary Medicine, Assiut University, 71526 Assiut, Egypt.
Pathogens. 2020 Apr 24;9(4):314. doi: 10.3390/pathogens9040314.
This report illustrates, for the first time, a case of unilateral orchitis and epididymitis in a Holstein-Friesian bull, associated with infection ( serovar Typhimurium). A one and a half-year-old Holstein-Friesian bull had arrived at the Veterinary Hospital of Assiut University suffering from anorexia accompanied with persistent fever, which did not respond to oxytetracycline and flunixin meglumine injection for 15 days. Gross examination revealed left scrotal enlargement (three times its normal size), heat sensation, and induration of the testis and epididymis, which was painful on external palpation. Microbiological and pathological examinations of the left testicle, epididymis, and spleen samples were performed. Typhimurium was recovered from the affected tissues and its critical virulence genes (, A and B) were identified. Pathological examination revealed a unilateral necrotizing intratubular pyogranulomatus orchitis and epididymitis with severe peri-orchitis. In addition, splenomegaly with a firm and large whitish nodular capsular structure associated with different stages of granulomatous reaction around the white and red pulp. To the authors' knowledge, this report is the first isolation of Typhimurium from the epididymis and testicles of a Holstein-Friesian bull. These results highlight the importance of including Typhimurium among the health disorders associated with stressful situations in bovine with orchitis and or/epididymitis. In Egypt, infection as being enzootic with high probability of dissemination should be considered one of genital health problems among cattle farms.
本报告首次阐述了一例荷斯坦-弗里生公牛单侧睾丸炎和附睾炎病例，与感染（鼠伤寒血清型）有关。一头一岁半的荷斯坦-弗里生公牛被送到阿斯尤特大学兽医院，患有厌食症并伴有持续发热，对土霉素和氟尼辛葡甲胺注射治疗15天无反应。大体检查发现左侧阴囊肿大（为正常大小的三倍）、有热感，睾丸和附睾硬结，外部触诊时有疼痛感。对左侧睾丸、附睾和脾脏样本进行了微生物学和病理学检查。从受影响的组织中分离出鼠伤寒杆菌，并鉴定出其关键毒力基因（、A和B）。病理检查显示单侧坏死性管内脓性肉芽肿性睾丸炎和附睾炎，并伴有严重的睾丸周围炎。此外，脾脏肿大，有一个坚实的、大的白色结节状包膜结构，与白髓和红髓周围不同阶段的肉芽肿反应有关。据作者所知，本报告是首次从荷斯坦-弗里生公牛的附睾和睾丸中分离出鼠伤寒杆菌。这些结果凸显了在与患有睾丸炎和/或附睾炎的牛的应激情况相关的健康疾病中纳入鼠伤寒杆菌的重要性。在埃及，感染作为一种具有高传播可能性的地方病，应被视为养牛场中的生殖健康问题之一。
