Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu, China.
J Biomater Sci Polym Ed. 2020 Aug;31(11):1421-1436. doi: 10.1080/09205063.2020.1760700. Epub 2020 May 13.
A novel bilayer fibrous membrane for guided tissue regeneration (GTR) was prepared two-step electrospinning process, subsequent crosslinking and surface conjugation with heparin. The bilayer membrane consists of upper layer polycaprolactone/gelatin (PCL/Gel) membrane for soft tissue regeneration and lower layer PCL/Gel/nano-hydroxyapatite (PCL/Gel/n-HA) membrane for hard tissue regeneration. The results indicated that the physicochemical and biological properties of the membrane were strongly influenced by the crosslinking time and by the heparin conjugation. Crosslinking effectively prolonged the degradation time while maintaining the membrane barrier function, and the surface heparin conjugation obviously improved the biological performance of the membrane. An enhanced cell adhesion and proliferation were observed on the heparin-conjugated fibrous membranes, which also showed good histocompatibility and favorable degradability. The electrospun bilayer fibrous membrane may have promising prospect for modulation of cell response and simultaneous regeneration of soft and hard tissues.
一种用于引导组织再生（GTR）的新型双层纤维膜，采用两步静电纺丝工艺，随后进行交联和肝素表面接枝。双层膜由上层聚己内酯/明胶（PCL/Gel）膜用于软组织再生和下层 PCL/Gel/纳米羟基磷灰石（PCL/Gel/n-HA）膜用于硬组织再生组成。结果表明，膜的物理化学和生物学性质受交联时间和肝素接枝的强烈影响。交联有效地延长了降解时间，同时保持了膜的屏障功能，而表面肝素接枝明显改善了膜的生物学性能。肝素接枝纤维膜上观察到细胞黏附和增殖增强，具有良好的组织相容性和可降解性。静电纺双层纤维膜可能具有调节细胞反应和同时再生软组织和硬组织的广阔前景。
