National Center for International Research of Micro-Nano Molding Technology, Zhengzhou University, Zhengzhou, China; School of Mechanics and Engineering Science, Zhengzhou University, Zhengzhou, China; Department of Mechanical Engineering, University of Wisconsin-Madison, WI, USA; Wisconsin Institute for Discovery, University of Wisconsin-Madison, WI, USA.
National Center for International Research of Micro-Nano Molding Technology, Zhengzhou University, Zhengzhou, China; Department of Mechanical Engineering, University of Wisconsin-Madison, WI, USA; Wisconsin Institute for Discovery, University of Wisconsin-Madison, WI, USA.
Mater Sci Eng C Mater Biol Appl. 2017 Feb 1;71:901-908. doi: 10.1016/j.msec.2016.10.083. Epub 2016 Nov 2.
During the fabrication of tissue engineering scaffolds and subsequent tissue regeneration, surface bioactivity is vital for cell adhesion, spreading, and proliferation, especially for endothelium dysfunction repair. In this paper, synthetic polymer polycaprolactone (PCL) was blended with natural polymer gelatin at four different weight ratios followed by crosslinking (i.e., 100:0, 70:30, 50:50, 30:70, labeled as PCL-C, P7G3-C, P5G5-C, and P3G7-C) to impart enhanced bioactivity and tunable mechanical properties. The PCL/gelatin blends were first dissolved in 2,2,2-trifluroethanol (TFE) and supplementary acetic acid (1% relative to TFE) solvent, electrospun, and then cross-linked to produce PBS-proof fibrous scaffolds. Scanning electron micrographs (SEM) indicated that fibers of each sample were smooth and homogeneous, with the fiber diameters increasing from 1.01±0.51μm to 1.61±0.46μm as the content of gelatin increased. While thermal resistance and crystallization of the blends were affected by the presence of gelatin, as reflected by differential scanning calorimetry (DSC) results, water contact angle (WCA) tests confirmed that the scaffold surfaces became more hydrophilic. Tensile tests showed that PCL-C and P7G3-C scaffolds had mechanical properties comparable to those of human coronary arteries. As for cytocompatibility, skeleton staining images showed that human mesenchymal stem cells (hMSCs) had more favorable binding sites on PCL/gelatin scaffolds than those on PCL scaffolds. Cell proliferation assays revealed that P7G3-C scaffolds could support the most number of hMSCs. The results of this study demonstrated the enhanced cell-matrix interactions and potential use of electrospun PCL/gelatin scaffolds in the tissue engineering field, especially in wound dressings and endothelium regeneration.
在组织工程支架的制造和随后的组织再生过程中，表面生物活性对于细胞黏附、铺展和增殖至关重要，特别是对于内皮功能障碍的修复。在本文中，合成聚合物聚己内酯（PCL）与天然聚合物明胶以四种不同的重量比混合，随后进行交联（即 100:0、70:30、50:50、30:70，分别标记为 PCL-C、P7G3-C、P5G5-C 和 P3G7-C），以赋予增强的生物活性和可调的机械性能。PCL/明胶共混物首先溶解在 2,2,2-三氟乙醇（TFE）和补充的乙酸（相对于 TFE 为 1%）溶剂中，然后进行静电纺丝和交联，以生产耐 PBS 的纤维状支架。扫描电子显微镜（SEM）表明，每个样品的纤维都光滑均匀，随着明胶含量的增加，纤维直径从 1.01±0.51μm 增加到 1.61±0.46μm。尽管存在明胶会影响共混物的热阻和结晶，但差示扫描量热法（DSC）结果表明，水接触角（WCA）测试证实支架表面变得更加亲水。拉伸试验表明，PCL-C 和 P7G3-C 支架的机械性能与人体冠状动脉相当。至于细胞相容性，骨骼染色图像表明，人骨髓间充质干细胞（hMSCs）在 PCL/明胶支架上比在 PCL 支架上具有更有利的结合部位。细胞增殖试验表明，P7G3-C 支架可以支持最多数量的 hMSCs。这项研究的结果表明，静电纺丝 PCL/明胶支架增强了细胞-基质相互作用，具有在组织工程领域的潜在应用，特别是在伤口敷料和内皮再生方面。
