Division of Bioinformatics, Bose Institute, Kolkata, India.
J Biosci. 2020;45.
Pluripotency in stem cells is regulated by a complex network between the transcription factors, signaling molecules, mRNAs, and epigenetic regulators like non-coding RNAs. Different pluripotent stem cell (PSC) lines were isolated and characterized to study the regulatory network topology to understand the mechanism that control developmental potential of pluripotent cells. PSCRIdb is a manually curated database of regulatory interactions including protein-protein, protein-DNA, gene-gene, and miRNA-mRNA interactions in mouse and human pluripotent stem cells including embryonic stem cells and embryonic carcinoma cells. At present, 22 different mouse and human pluripotent stem-cell-line-specific regulatory interactions are compiled in the database. Detailed information of the four types of interaction data are presented in tabular format and graphical network view in Cytoscape layout. The database is available at http://bicresources.jcbose.ac.in/ ssaha4/pscridb. The database contains 3037 entries of experimentally validated molecular interactions that can be useful for systematic study of pluripotency integrating multi-omics data. In summary, the database can be a useful resource for identification of regulatory networks present in different pluripotent stem cell lines.
多能性在干细胞中由转录因子、信号分子、mRNA 以及表观遗传调节剂（如非编码 RNA）之间的复杂网络调控。为了研究调控网络拓扑结构，以了解控制多能细胞发育潜能的机制，分离并鉴定了不同的多能干细胞（PSC）系。PSCrdb 是一个经过人工整理的数据库，其中包含了调控相互作用，包括在小鼠和人类多能干细胞（包括胚胎干细胞和胚胎癌细胞）中蛋白-蛋白、蛋白-DNA、基因-基因和 miRNA-mRNA 相互作用。目前，该数据库中编译了 22 种不同的小鼠和人类多能干细胞系特异性调控相互作用。四种类型的交互数据的详细信息以表格格式和 Cytoscape 布局的图形网络视图呈现。该数据库可在 http://bicresources.jcbose.ac.in/ssaha4/pscridb 上获得。该数据库包含 3037 条经过实验验证的分子相互作用条目，这些条目可用于整合多组学数据对多能性进行系统研究。总之，该数据库可以成为鉴定不同多能干细胞系中存在的调控网络的有用资源。
