Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
Chem Commun (Camb). 2020 May 18;56(40):5358-5361. doi: 10.1039/d0cc01865a. Epub 2020 Apr 29.
A triplex-forming oligonucleotide (TFO) linear probe containing perylene derivatives was synthesized. The TFO linear probe formed a remarkably stable triplex with a target DNA duplex, resulting in the light-up of fluorescence emission. The sensitivity was extremely high even at pH 7. Detection of PCR-amplified target DNA was demonstrated.
合成了一种含有苝衍生物的三聚体形成寡核苷酸（TFO）线性探针。该 TFO 线性探针与靶 DNA 双链形成了非常稳定的三聚体，导致荧光发射增强。即使在 pH 7 时，灵敏度也非常高。还证明了对 PCR 扩增靶 DNA 的检测。
