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实验室条件下黄斑鸟蛤(Mesodesma mactroides (Reeve, 1854),鸟蛤科)的胚胎和幼虫发育

Embryo and larval development of the yellow clam Mesodesma mactroides (Reeve, 1854) (Mesodesmatidae) in laboratory.

作者信息

Santos Juan J S, Bernardes Juliana P, Ramírez Juan R B, Ramos Cássio O, Gomes Carlos Henrique A DE Miranda, Romano Luis Alberto

机构信息

Laboratório de Imunologia e Patologia de Organismos Aquáticos/LIPOA, Universidade Federal do Rio Grande, Rio Grande, RS, Brazil.

Laboratório de Moluscos Marinhos/LMM, Universidade Federal de Santa Catarina/UFSC, Florianópolis, SC, Brazil.

出版信息

An Acad Bras Cienc. 2020;92 Suppl 1:e20190053. doi: 10.1590/0001-3765202020190053. Epub 2020 Apr 27.

DOI:10.1590/0001-3765202020190053
PMID:32348413
Abstract

The yellow clam Mesodesma mactroides (Reeve, 1854) is a sand mollusc with historical and socioeconomic importance in Brazil, Uruguay and Argentina. A guaranteed form to access a successful reestablishment of the species in their natural environment is directly linked to their reproduction biology. Then, our report introduces the embryonic and larval development of the yellow clam reared in laboratory for such purposes. M. mactroides broodstock were selected as specimens who possess a mean total shell length and weight of 66 ± 3.82 mm and 27.15 ± 4.07 g for an afterwards spawn induction through stripping technique. Regarding the embryonic development, newly fertilized oocytes exhibited a mean diameter of 51.20 ± 6.64 μm. The first polar corpuscle, trochophores and D-veliger appeared at 20 min, 18 and 24 h after fertilization, respectively. Umbonate and pediveliger larvae were noticed on the 8th and 25th day, respectively, with complete metamorphosis occurring only at the 27th day, when all larvae were retained in a 200 μm nylon mesh. Therefore, with that basic understanding of the embryonic and larval development of M. mactroides in the laboratory, forwards studies will focus in establish a technological package for this species.

摘要

黄斑鸟蛤(Mesodesma mactroides,里夫,1854年)是一种生活在沙滩的软体动物,在巴西、乌拉圭和阿根廷具有重要的历史和社会经济价值。确保该物种在自然环境中成功重新建立种群的一个关键因素直接与其繁殖生物学相关。因此,我们的报告介绍了为此目的在实验室中饲养的黄斑鸟蛤的胚胎和幼虫发育情况。选择黄斑鸟蛤亲体作为样本,其平均总壳长和体重分别为66 ± 3.82毫米和27.15 ± 4.07克,随后通过解剖技术诱导产卵。关于胚胎发育,新受精的卵母细胞平均直径为51.20 ± 6.64微米。第一极体、担轮幼虫和D形幼虫分别在受精后20分钟、18小时和24小时出现。分别在第8天和第25天观察到具壳幼虫和足面盘幼虫,只有在第27天所有幼虫都留在200微米尼龙网中时才发生完全变态。因此,基于对实验室中黄斑鸟蛤胚胎和幼虫发育的基本了解,未来的研究将集中于为该物种建立一套技术方案。

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引用本文的文献

1
Effect of Sperm Ratio and Temperature on Fertilization and Early Larval Development of the Surf Clam (Bivalvia:Mesodesmatidae).精子比例和温度对 surf 蛤(双壳纲:中带蛤科)受精及幼体早期发育的影响
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