Nanjing Normal University, School of Environment, Wenyuan Road 1, Nanjing 210023, China; Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, School of Geography Science, Nanjing Normal University, Nanjing 210023, China; Jiangsu Provincial Key Laboratory of Materials Cycling and Pollution Control, Nanjing Normal University, Nanjing 210023, China.
Nanjing Normal University, School of Environment, Wenyuan Road 1, Nanjing 210023, China.
Sci Total Environ. 2020 Aug 1;728:138615. doi: 10.1016/j.scitotenv.2020.138615. Epub 2020 Apr 19.
The bacterial phoD gene encodes alkaline phosphatase plays an important role in the release of bioavailable inorganic phosphorus (P) from organic P in environmental systems. However, phoD gene diversity in suspended particles in shallow freshwater lakes is poorly understood. In this study, we explored the potential relationship between environmental factors and phoD phosphatase gene in suspended particles in different ecosystem types (lake zones) in Lake Taihu, a large shallow eutrophic lake in China. Quantitative PCR and high-throughput sequencing were used to analyze phoD gene abundance and the phoD-harboring bacterial community composition. Our results indicate that the distribution of phoD gene abundance in suspended particles had a high spatiotemporal heterogeneity. The phoD gene abundance in each lake zone decreased significantly from June to September. The dominant phoD-harboring phylum in all samples was Actinobacteria, followed by Proteobacteria, Cyanobacteria and Gemmatimonadetes. The first predominant phoD-harboring genera varied among samples, but most of them belonged to phylum Actinobacteria. Driven by different environmental factors, the phoD-harboring bacterial community structure varied with sampling month and ecosystem type. Nitrate and ammonia nitrogen were the main environmental drivers of phoD-harboring bacterial community in suspended particles in the river mouth zone, while water pH and dissolved oxygen were important factors for the algae-dominated, macrophyte-dominated and central lake zones.
细菌 phoD 基因编码碱性磷酸酶，在环境系统中从有机磷释放可利用的无机磷 (P) 方面发挥着重要作用。然而，浅水湖泊悬浮颗粒中 phoD 基因多样性的了解甚少。在这项研究中，我们探讨了中国太湖大型浅水富营养湖中不同生态系统类型（湖带）悬浮颗粒中环境因子与 phoD 磷酸酶基因之间的潜在关系。使用定量 PCR 和高通量测序分析了 phoD 基因丰度和携带 phoD 的细菌群落组成。我们的结果表明，悬浮颗粒中 phoD 基因丰度的分布具有高度的时空异质性。每个湖带的 phoD 基因丰度从 6 月到 9 月显著下降。所有样本中占主导地位的 phoD 携带门是放线菌门，其次是变形菌门、蓝细菌门和芽单胞菌门。所有样本中占主导地位的 phoD 携带属各不相同，但大多数属于放线菌门。受不同环境因素的驱动，携带 phoD 的细菌群落结构随采样月份和生态系统类型而变化。硝酸盐和氨氮是河口带悬浮颗粒中携带 phoD 的细菌群落的主要环境驱动因素，而水 pH 值和溶解氧是藻类占主导、大型水生植物占主导和中心湖区的重要因素。
