Dai Jiangyu, Chen Dan, Wu Shiqiang, Wu Xiufeng, Zhou Jie, Tang Xiangming, Shao Keqiang, Gao Guang
State Key Laboratory of Hydrology - Water Resources and Hydraulic Engineering, Nanjing Hydraulic Research Institute, Nanjing 210029, People's Republic of China.
Can J Microbiol. 2015 Mar;61(3):227-36. doi: 10.1139/cjm-2014-0446. Epub 2014 Dec 22.
Limnetic habitats that are dominated by either algae or macrophytes represent the 2 dominant ecosystems in shallow lakes. We assessed seasonal variations in the diversity and abundance of alkaline phosphate-encoding genes (phoX) in these 2 zones of Lake Taihu, which is a large, shallow, eutrophic lake in China. There was no significant difference in seasonal mean phoX diversity between the 2 zones, whereas the seasonal mean phoX abundance in the macrophyte-dominated region was higher than that in the algae-dominated region. The bulk of the genotypes in the 2 regions were most similar to the alphaproteobacterial and betaproteobacterial phoX. Genotypes most similar to phoX affiliated with Betaproteobacteria were present with greater diversity in the macrophyte-dominated zone than in the algae-dominated zone. In the algae-dominated zone, the relative proportion of genotypes most similar to cyanobacterial phoX was highest (38.8%) in summer. In addition to the different genotype structures and environmental factors between the 2 stable states, the lower gene abundances and higher alkaline phosphatase activities in Meiliang Bay in summer than those in Xukou Bay reveals different organophosphate-mineralizing modes in these 2 contrasting habitats.
以藻类或大型植物为主的敞水区代表了浅水湖泊中的两种主要生态系统。我们评估了中国大型浅水富营养化湖泊太湖这两个区域中碱性磷酸酶编码基因（phoX）多样性和丰度的季节变化。这两个区域的季节性平均phoX多样性没有显著差异，而大型植物为主的区域季节性平均phoX丰度高于藻类为主的区域。这两个区域的大部分基因型与α-变形菌纲和β-变形菌纲的phoX最为相似。与β-变形菌纲相关的phoX最相似的基因型在大型植物为主的区域比在藻类为主的区域具有更高的多样性。在藻类为主的区域，与蓝细菌phoX最相似的基因型的相对比例在夏季最高（38.8%）。除了这两种稳定状态之间不同的基因型结构和环境因素外，夏季梅梁湾的基因丰度低于胥口湾，碱性磷酸酶活性高于胥口湾，这揭示了这两个截然不同生境中不同的有机磷矿化模式。
