Miller B J, Graves C N, Lodge J R
Department of Animal Sciences, University of Illinois, Urbana.
Gamete Res. 1988 May;20(1):53-65. doi: 10.1002/mrd.1120200106.
During a 10-day 5 degrees C storage and subsequent 4-6-hr 37 degrees C incubation, both percent live spermatozoa and percent spermatozoa with an intact acrosome decreased, and percent spermatozoa with a late-reacted or without an acrosome increased. When stored spermatozoa were mixed with oocytes, no decrease in percent of oocytes with spermatozoa bound or percent of oocytes with spermatozoa starting to penetrate occurred as storage time increased. A 58% decrease in acrosin gelatinolytic activity and a 56% decrease in acrosin esterolytic activity but no decrease in nonacrosin proteolytic activity were evident over the 10-day storage. These studies show that a change in acrosomal morphology as well as a loss of acrosin may be responsible for the decreased fertility following extended in vitro storage of mammalian spermatozoa.
在10天5摄氏度的储存以及随后4至6小时37摄氏度的孵育过程中，活精子百分比和顶体完整的精子百分比均下降，而发生晚期反应或无顶体的精子百分比增加。当将储存的精子与卵母细胞混合时，随着储存时间的增加，与精子结合的卵母细胞百分比或开始穿透精子的卵母细胞百分比均未下降。在10天的储存过程中，顶体蛋白酶解活性下降了58%，顶体酯酶解活性下降了56%，但非顶体蛋白水解活性没有下降。这些研究表明，顶体形态的改变以及顶体蛋白酶的丧失可能是哺乳动物精子体外长时间储存后生育力下降的原因。
