Department of Fundamental and Applied Sciences, HICoE-Centre for Biofuel and Biochemical Research, Institute of Self-Sustainable Building, Universiti Teknologi PETRONAS, 32610, Seri Iskandar, Perak Darul Ridzuan, Malaysia.
Department of Chemical Engineering, HICoE-Centre for Biofuel and Biochemical Research, Institute of Self-Sustainable Building, Universiti Teknologi PETRONAS, 32610, Seri Iskandar, Perak Darul Ridzuan, Malaysia.
Environ Sci Pollut Res Int. 2020 Jul;27(19):24574-24581. doi: 10.1007/s11356-020-09034-2. Epub 2020 Apr 29.
The anaerobic decomposition of coconut endosperm waste (CEW), residue derived from cooking, has been insidiously spewing greenhouse gasses. Thus, the bioconversion of CEW via in situ fermentation by exo-microbes from commercial Rid-X and subsequent valorization by black soldier fly larvae (BSFL) was the primary objective of the current study to gain sustainable larval lipid and protein. Accordingly, various concentrations of exo-microbes were separately homogenized with CEW to perform fermentation amidst feeding to BSFL. It was found that 2.50% of exo-microbes was the threshold amount entailed to assuage competition between exo-microbes and BSFL for common nutrients. The presence of remnant nutrients exuded from the fermentation using 2.50% of exo-microbes was confirmed to promote BSFL growth measured as maximum larval weight gained and growth rate. Although the BSFL could accumulate the highest protein (16 mg/larva) upon feeding with CEW containing 2.50% of exo-microbes, more lipid (13 mg/larva) was stored in employing 0.10% of exo-microbes because of minimum loss to metabolic processes while prolonging the BSFL in its 5th instar stage.
椰子胚乳废物（CEW）是烹饪后的剩余物，其厌氧分解一直在悄然排放温室气体。因此，本研究的主要目的是通过商业 Rid-X 的胞外微生物进行原位发酵来生物转化 CEW，然后利用黑水虻幼虫（BSFL）对其进行增值，以获得可持续的幼虫脂质和蛋白质。为此，将不同浓度的胞外微生物分别与 CEW 均匀混合，在 BSFL 喂养过程中进行发酵。结果发现，2.50%的胞外微生物是缓解胞外微生物和 BSFL 之间竞争共同营养物质所必需的阈值量。使用 2.50%的胞外微生物进行发酵后，剩余营养物质的存在得到了证实，这促进了 BSFL 的生长，表现为最大幼虫体重增加和生长速度。尽管 BSFL 在喂食含有 2.50%胞外微生物的 CEW 时可以积累最高的蛋白质（16 毫克/只幼虫），但在使用 0.10%胞外微生物时，储存的脂质更多（13 毫克/只幼虫），因为在延长 BSFL 进入第 5 龄期的同时，代谢过程中的损失最小。
