Department of Pharmacy, Affiliated Zhongshan Hospital of Dalian University, Dalian, P. R. China.
Institute of Wudang Traditional Chinese Medicine, Taihe Hospital, Hubei University of Medicine, Shiyan, P. R. China.
Electrophoresis. 2020 Jul;41(13-14):1253-1260. doi: 10.1002/elps.202000003. Epub 2020 May 25.
In this work, we utilized adsorbed BSA and multiwalled carbon nanoparticles (BSA/MWCNTs) as a stationary phase in open tubular (OT) capillary for separation of chiral drugs. (3-Aminopropyl)triethoxysilane was used to assist fabrication of BSA/MWCNTs-coated OT column by covalent bonding. Incorporation of MWCNTs nanomaterials into a polymer matrix could increase the phase ratio and take advantage of the easy preparation of an open tubular CEC column. SEM was carried out to characterize the BSA/MWCNTs OT columns. The electrochromatographic performance of the OT columns was evaluated by separation of ketoprofen, ibuprofen, uniconazole, and hesperidin. The effects of MWCNTs concentration, background solution pH and concentration, and applied voltage on separation were investigated. Chiral separations of ketoprofen, ibuprofen, uniconazole, and hesperidin were achieved using the BSA/MWCNTs-coated OT column with resolutions of 24.20, 12.81, 1.50, and 1.85, respectively. Their optimas were found in the 30 mM phosphate buffers at pH 5.0, 6.5, 7.0, and 6.5, respectively. In addition, the columns demonstrated good repeatability and stability with the run-to-run, day-to-day, and batch-to-batch RSDs of migration times less than 3.5%.
在这项工作中，我们利用吸附的牛血清白蛋白和多壁碳纳米粒子（BSA/MWCNTs）作为开管（OT）毛细管中的固定相，用于分离手性药物。（3-氨丙基）三乙氧基硅烷用于通过共价键合辅助制造 BSA/MWCNTs 涂层 OT 柱。将 MWCNTs 纳米材料掺入聚合物基质中可以增加相比例，并利用易于制备开管 CEC 柱的优势。通过 SEM 对 BSA/MWCNTs OT 柱进行了表征。通过分离酮洛芬、布洛芬、烯效唑和橙皮苷来评估 OT 柱的电色谱性能。研究了 MWCNTs 浓度、背景溶液 pH 值和浓度以及施加电压对分离的影响。使用 BSA/MWCNTs 涂层 OT 柱实现了酮洛芬、布洛芬、烯效唑和橙皮苷的手性分离，分辨率分别为 24.20、12.81、1.50 和 1.85。它们的最佳条件分别为 pH 5.0、6.5、7.0 和 6.5 的 30 mM 磷酸盐缓冲液。此外，该柱在运行到运行、每天和批到批的迁移时间 RSD 小于 3.5%的情况下表现出良好的重复性和稳定性。
