Shenzhen Key Laboratory for Endogenous Infection, Department of Infectious Diseases, Shenzhen Nanshan People's Hospital and the 6th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, China.
BGI Education Center, BGI Genomics and BGI-Shenzhen, University of Chinese Academy of Sciences, Shenzhen, China.
Front Public Health. 2020 Apr 15;8:121. doi: 10.3389/fpubh.2020.00121. eCollection 2020.
Two rare cases of Chinese female patients with influenza B virus infection complicated with both fulminant pneumonia and septicemia caused by Panton-Valentine leukocidin(PVL) positive methicillin-sensitive (MASS) were reported for the first time in China through next-generation sequencing (NGS). An increasing body of evidence indicates that co-infection with influenza B virus and bacterial pneumonia is often fatal. Rapid and precise identification of the co-infection bacteria can guide the selection of treatment for patients with influenza virus infection in the clinical setting. In this study, next-generation sequencing (NGS) was applied for the rapid diagnosis of these two cases. Despite the unfavorable survival outcome of these patients, the application of next-generation sequencing showed promise as a diagnostic tool for the rapid diagnosis of unknown pathogens in patients with bacterial pneumonia and sepsis. This method can guide the administration of medications in such patients.
本文首次通过下一代测序（NGS）在中国报道了两例罕见的乙型流感病毒感染合并由泛-潘顿-瓦伦丁白细胞素（PVL）阳性甲氧西林敏感（MSSA）引起的暴发性肺炎和败血症的中国女性患者。越来越多的证据表明，乙型流感病毒与细菌性肺炎的合并感染通常是致命的。快速准确地鉴定合并感染的细菌可以指导临床中流感病毒感染患者的治疗选择。在本研究中，应用下一代测序（NGS）快速诊断了这两例病例。尽管这些患者的生存结果不佳，但下一代测序的应用作为一种诊断工具，对于快速诊断细菌性肺炎和败血症患者的未知病原体具有应用前景。该方法可以指导此类患者的药物治疗。
