Sachs V, Dörner R, Rehder V
Abteilung Transfusionsmedizin, Klinikum Kiel.
Infusionstherapie. 1988 Dec;15(6):240-3.
The question of how often erythrocytes should be washed in order to obtain a protein-free and therapeutically efficient unit of washed red blood cells is still controversial. To obtain more information regarding this problem, 6 X 15 units of whole blood were washed 1-6 times with a saline solution immediately and after 7, 14, 21, 28 and 35 days of storage. After each washing procedure the content of total protein, of immunglobulins A, G, and M and of complement factor C3 was determined in the supernatants, as well as the osmotic fragility in the corresponding erythrocyte sediments. The statistically analyzed results enabled us to describe the average degree of influences as equations and to recommend a preparation prescription. The results show that the protein levels decrease precipitously after the first and second washing procedures. The following 4 procedures have no significant effect. On the other hand, with increasing time of storage more washing procedures are necessary to reach the same results than with younger units. That is also true for the concentrations of immunoglobulins and complement C3. The osmotic fragility of the erythrocytes increases with each washing procedure as well as each day of storage. According to these results, an optimal, protein-free and effective unit of washed red blood cells is obtained when a 1-2-week-old unit of whole blood is washed 2-3 times.
为了获得无蛋白且具有治疗效果的洗涤红细胞单位，红细胞应洗涤的频率问题仍存在争议。为了获取更多关于此问题的信息，对6×15单位的全血在储存1、7、14、21、28和35天后立即用盐溶液洗涤1至6次。每次洗涤后，测定上清液中总蛋白、免疫球蛋白A、G和M以及补体因子C3的含量，以及相应红细胞沉淀物中的渗透脆性。经统计分析的结果使我们能够用方程式描述平均影响程度并推荐制备处方。结果表明，在第一次和第二次洗涤后蛋白质水平急剧下降。接下来的4次洗涤没有显著影响。另一方面，随着储存时间的增加，与较新鲜的单位相比，需要更多的洗涤程序才能达到相同的结果。免疫球蛋白和补体C3的浓度也是如此。红细胞的渗透脆性随着每次洗涤以及储存的每一天而增加。根据这些结果，当洗涤1至2周龄的全血单位2至3次时，可获得最佳的、无蛋白且有效的洗涤红细胞单位。
