Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Ave. Eugenio Garza Sada 2501, Monterrey, NL C.P. 64849, Mexico.
Department of Horticultural Sciences, Texas A&M University, College Station, TX 77843-2133, USA.
Int J Mol Sci. 2020 Apr 28;21(9):3108. doi: 10.3390/ijms21093108.
Nutraceutical combinations that act synergistically could be a powerful solution against colon cancer, which is the second deadliest malignancy worldwide. In this study, curcumin (C), sulforaphane (S), and dihydrocaffeic acid (D, a chlorogenic acid metabolite) were evaluated, individually and in different combinations, over the viability of HT-29 and Caco-2 colon cancer cells, and compared against healthy fetal human colon (FHC) cells. The cytotoxic concentrations to kill 50%, 75%, and 90% of the cells (CC, CC, and CC) were obtained, using the MTS assay. Synergistic, additive, and antagonistic effects were determined by using the combination index (CI) method. The 1:1 combination of S and D exerted synergistic effects against HT-29 at 90% cytotoxicity level (doses 90:90 µM), whereas CD(1:4) was synergistic at all cytotoxicity levels (9:36-34:136 µM) and CD(9:2) at 90% (108:24 µM) against Caco-2 cells. SD(1:1) was significantly more cytotoxic for cancer cells than healthy cells, while CD(1:4) and CD(9:2) were similarly or more cytotoxic for healthy cells. Therefore, the SD(1:1) combination was chosen as the best. A model explaining SD(1:1) synergy is proposed. SD(1:1) can be used as a basis to develop advanced food products for the prevention/co-treatment of colon cancer.
具有协同作用的营养组合可能是对抗全球第二大致命恶性肿瘤结肠癌的有力解决方案。在这项研究中，评估了姜黄素（C）、萝卜硫素（S）和二氢咖啡酸（D，绿原酸的代谢物），单独使用和不同组合使用，以评估其对 HT-29 和 Caco-2 结肠癌细胞活力的影响，并与健康胎儿人结肠（FHC）细胞进行了比较。使用 MTS 测定法获得了杀死 50%、75%和 90%的细胞（CC、CC 和 CC）的细胞毒性浓度。通过使用组合指数（CI）方法确定协同、相加和拮抗作用。S 和 D 的 1:1 组合在 90%细胞毒性水平（剂量 90:90 µM）下对 HT-29 表现出协同作用，而 CD（1:4）在所有细胞毒性水平（9:36-34:136 µM）和 CD（9:2）在 90%（108:24 µM）对 Caco-2 细胞表现出协同作用。SD（1:1）对癌细胞的细胞毒性明显高于健康细胞，而 CD（1:4）和 CD（9:2）对健康细胞的细胞毒性相似或更高。因此，选择 SD（1:1）组合作为最佳选择。提出了一种解释 SD（1:1）协同作用的模型。SD（1:1）可以作为开发预防/联合治疗结肠癌的先进食品产品的基础。
