Faculty of Health Sciences, Nihon Institute of Medical Science, Saitama, Japan.
Meikai University School of Dentistry, Saitama, Japan
In Vivo. 2020 May-Jun;34(3):1009-1016. doi: 10.21873/invivo.11869.
In order to investigate the combination effect of anticancer drugs and X-ray irradiation on neurotoxic side-effects (neurotoxicity), a method that provides homogeneously X-ray-irradiated cells was newly established.
PC12 cell suspension was irradiated by X-ray (0.5 Gy) in serum-supplemented medium, immediately inoculated into 96-microwell plates and incubated overnight. The medium was replaced with fresh serum-depleted medium containing 50 ng/ml nerve growth factor to induce differentiation toward nerve-like cells with characteristic neurites according to the overlay method without changing the medium. The differentiated cells were treated by anticancer drugs as well as antioxidants, oxaliplatin or bortezomib, and the viable cell number was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method.
Antioxidants and anticancer drugs were cytotoxic to differentiating PC12 cells. Combination of anticancer drugs and X-ray irradiation slightly reduced cell viability.
The present 'population irradiation method' may be useful for the investigation of the combination effect of X-ray irradiation and any pharmaceutical drug.
为了研究抗癌药物与 X 射线照射联合对神经毒性（神经毒性）的影响，建立了一种能均匀照射细胞的方法。
将 PC12 细胞悬液在含血清的培养基中用 X 射线（0.5Gy）照射，立即接种到 96 孔板中培养过夜。用不含血清的培养基替换新鲜的培养基，其中含有 50ng/ml 神经生长因子，根据覆盖法诱导向具有特征性神经突的神经样细胞分化，无需更换培养基。用抗癌药物和抗氧化剂、奥沙利铂或硼替佐米处理分化的细胞，并用 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐法测定活细胞数。
抗氧化剂和抗癌药物对分化的 PC12 细胞具有细胞毒性。抗癌药物与 X 射线照射联合使用略微降低了细胞活力。
本研究中的“群体照射方法”可能有助于研究 X 射线照射与任何药物联合作用的效果。
