壳聚糖磁性纳米粒子聚集形式的生物学特性。
Biological Properties of the Aggregated Form of Chitosan Magnetic Nanoparticle.
机构信息
Laboratorio de Investigación Interdisciplinaria, Área de Nanoestructuras y Biomateriales, Escuela Nacional de Estudios Superiores Unidad León, León, México.
Dental Science, National Autonomous University of Mexico, Mexico City, Mexico.
出版信息
In Vivo. 2020 Jul-Aug;34(4):1729-1738. doi: 10.21873/invivo.11966.
BACKGROUND/AIM: Chitosan-coated iron oxide nanoparticles (Chi-NP) have gained attention because of their biocompatibility, biodegradability, low toxicity and targetability under magnetic field. In this study, we investigated various biological properties of Chi-NP.
MATERIALS AND METHODS
Chi-NP was prepared by mixing magnetic NP with chitosan FL-80. Particle size was determined by scanning and transmission electron microscopes, cell viability by MTT assay, cell cycle distribution by cell sorter, synergism with anticancer drugs by combination index, PGE production in human gingival fibroblast was assayed by ELISA.
RESULTS
The synthetic process of Chi-NP from FL-80 and magnetic NP increased the affinity to cells, up to the level attained by nanofibers. Upon contact with the culture medium, Chi-NP instantly formed aggregates and interfered with intracellular uptake. Aggregated Chi-NP did not show cytotoxicity, synergism with anticancer drugs, induce apoptosis (accumulation of subG1 cell population), protect the cells from X-ray-induced damage, nor affected both basal and IL-1β-induced PGE production.
CONCLUSION
Chi-NP is biologically inert and shows high affinity to cells, further confirming its superiority as a scaffold for drug delivery.
背景/目的：壳聚糖包覆的氧化铁纳米颗粒（Chi-NP）由于其生物相容性、可生物降解性、低毒性和在磁场下的靶向性而受到关注。在本研究中，我们研究了 Chi-NP 的各种生物学特性。
材料与方法
Chi-NP 通过将磁性 NP 与壳聚糖 FL-80 混合制备。通过扫描和透射电子显微镜确定粒径，通过 MTT 测定法测定细胞活力，通过细胞分选仪测定细胞周期分布，通过组合指数测定与抗癌药物的协同作用，通过 ELISA 测定人牙龈成纤维细胞中 PGE 的产生。
结果
从 FL-80 和磁性 NP 合成 Chi-NP 的过程增加了对细胞的亲和力，达到了纳米纤维的水平。与培养基接触后，Chi-NP 立即形成聚集体并干扰细胞内摄取。聚集的 Chi-NP 没有显示出细胞毒性、与抗癌药物的协同作用、诱导细胞凋亡（亚 G1 细胞群的积累）、保护细胞免受 X 射线诱导的损伤，也不影响基础和 IL-1β 诱导的 PGE 产生。
结论
Chi-NP 具有生物惰性，对细胞具有高亲和力，进一步证实了其作为药物输送支架的优越性。
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