大鼠脑中蛋氨酸脑啡肽和亮氨酸脑啡肽的同步分析：液相色谱-电化学法定量

Simultaneous analysis of methionine- and leucine-enkephalin from rat brain: quantification by liquid chromatography-electrochemistry.

作者信息

Fleming L H, Reynolds N C

机构信息

David I.M. Fine Memorial Neurology Research Laboratory, Department of Neurology University of Wisconsin Medical School, Sinai Samaritan Medical Center, Milwaukee 53201.

出版信息

J Chromatogr. 1988 Sep 23;431(1):65-76. doi: 10.1016/s0378-4347(00)83070-4.

DOI:10.1016/s0378-4347(00)83070-4

PMID:3235537

Abstract

This study focuses on the application of liquid chromatography with electrochemical detection (LC-ED) for the analysis of methionine-enkephalin (ME) and leucine-enkephalin (LE) extracted from rat brain regions. The high applied potentials necessary for enkephalin detection required the development of an efficient sample processing protocol. Brain extracts were processed using chromatographic mode sequencing (CMS). The decrease in electroactive interfering substances by CMS improved the chromatographic resolution of ME and LE and the electrode performance. Other qualitative and analytical methods were used to evaluate the enkephalin data obtained by LC-ED for rat brain regions. This study demonstrates that LC-ED provides both the sensitivity and specificity necessary for the analysis of enkephalins from rat brain regions.

摘要

本研究聚焦于液相色谱-电化学检测法（LC-ED）在分析从大鼠脑区提取的甲硫氨酸脑啡肽（ME）和亮氨酸脑啡肽（LE）中的应用。脑啡肽检测所需的高施加电位要求开发一种高效的样品处理方案。脑提取物采用色谱模式测序（CMS）进行处理。CMS降低了电活性干扰物质，提高了ME和LE的色谱分辨率以及电极性能。还使用了其他定性和分析方法来评估通过LC-ED获得的大鼠脑区脑啡肽数据。本研究表明，LC-ED为分析大鼠脑区的脑啡肽提供了所需的灵敏度和特异性。