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采用高效液相色谱-电化学检测法测定大鼠脑离散区域中的神经肽。

Determination of neuropeptides in discrete regions of the rat brain by high-performance liquid chromatography with electrochemical detection.

作者信息

Sauter A, Frick W

出版信息

J Chromatogr. 1984 Aug 3;297:215-23. doi: 10.1016/s0021-9673(01)89043-9.

DOI:10.1016/s0021-9673(01)89043-9
PMID:6490757
Abstract

High-performance liquid chromatography (HPLC) in combination with electrochemical detection (ED) is suitable for measuring accurately the levels of oxidizable neuropeptides in small samples of brain tissue. We have determined by HPLC-ED and a two-column-switching technique the levels of leucine- and methione-enkephalin in rat brain. By a sequential two-column HPLC-ED method we have also determined the levels of cholecystokinin tetrapeptide and octapeptide sulphate in various regions of the rat brain. The values found are in good agreement with those reported previously based on radioimmunoassay. We conclude that, due to its simplicity, HPLC-ED is an attractive alternative to existing methods for the determination of some neuropeptides in biological material.

摘要

高效液相色谱法（HPLC）结合电化学检测（ED）适用于精确测量脑组织小样本中可氧化神经肽的水平。我们已通过HPLC-ED和双柱切换技术测定了大鼠脑中亮氨酸脑啡肽和甲硫氨酸脑啡肽的水平。通过连续双柱HPLC-ED方法，我们还测定了大鼠脑不同区域中胆囊收缩素四肽和八肽硫酸盐的水平。所获得的值与先前基于放射免疫测定法报道的值高度一致。我们得出结论，由于其简便性，HPLC-ED是测定生物材料中某些神经肽的现有方法的一种有吸引力的替代方法。

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1
Determination of neuropeptides in discrete regions of the rat brain by high-performance liquid chromatography with electrochemical detection.采用高效液相色谱-电化学检测法测定大鼠脑离散区域中的神经肽。
J Chromatogr. 1984 Aug 3;297:215-23. doi: 10.1016/s0021-9673(01)89043-9.
2
Determination of cholecystokinin tetrapeptide and cholecystokinin octapeptide sulfate in different rat brain regions by high-pressure liquid chromatography with electrochemical detection.采用高效液相色谱-电化学检测法测定不同大鼠脑区中的胆囊收缩素四肽和硫酸化胆囊收缩素八肽。
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Quantitation and identification of two cholecystokinin peptides, CCK-4 and CCK-8s, in rat brain by HPLC and fast atom bombardment mass spectrometry.采用高效液相色谱法和快原子轰击质谱法对大鼠脑中两种胆囊收缩素肽CCK - 4和CCK - 8s进行定量和鉴定。
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Multi-dimensional high-performance liquid chromatography of opioid peptides following pre-column derivatization with naphthalene-2,3-dicarboxaldehyde in the presence of cyanide ion. Preliminary results on the determination of leucine- and methionine-enkephalin-like fluorescence in the striatum region of the rat brain.在氰离子存在下用萘-2,3-二甲醛进行柱前衍生化后阿片肽的多维高效液相色谱法。大鼠脑纹状体区域中亮氨酸脑啡肽和甲硫氨酸脑啡肽样荧光测定的初步结果。
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