School of Food Science and Biotechnology, Kyungpook National University, Daegu, 41566, Korea.
Department of Public Health Sciences, Graduate School, Korea University, Seoul, Korea.
Biotechnol Lett. 2020 Sep;42(9):1755-1766. doi: 10.1007/s10529-020-02899-w. Epub 2020 Apr 30.
Microbial bioconversion of a given substrate is considered an efficient and eco-friendly tool for value-added industrial compound generation from natural products. Among natural products, unsaturated fatty acids have been used as substrates to produce various functional hydroxy fatty acids. In this study, we report the production of 7,10-dihydroxy-8(E)-hexadecenoic acid (DHD) from palmitoleic acid by a new strain, Pseudomonas aeruginosa KNU-2B. KNU-2B efficiently produced DHD from palmitoleic acid and required a strong alkaline condition for maximum DHD production. The maximum DHD amount produced under pH 10.0 and 48-h incubation at 27 °C and 150 rpm was 219.5 mg/100 mL culture. Other important nutritional factors were also investigated to obtain optimum DHD production.
将给定的底物进行微生物生物转化被认为是一种从天然产物中生成附加值工业化合物的有效且环保的工具。在天然产物中，不饱和脂肪酸已被用作底物来生产各种功能性羟基脂肪酸。在本研究中，我们报告了一种新菌株铜绿假单胞菌 KNU-2B 从棕榈油酸生产 7,10-二羟基-8(E)-十六碳烯酸（DHD）的情况。KNU-2B 能够从棕榈油酸高效生产 DHD，并且需要强碱条件才能达到最大 DHD 产量。在 pH 值 10.0 下，27°C 和 150rpm 孵育 48 小时，最大 DHD 产量为 219.5mg/100mL 培养物。还研究了其他重要营养因素以获得最佳 DHD 产量。
