采用经溴化十六烷基三甲基铵预处理的C18柱，通过高效液相色谱法测定尿液中的蝶呤。

Determination of pterins in urine by high-performance liquid chromatography on C18 columns conditioned with cetyltrimethylammonium bromide.

作者信息

Montalvo B C, Villar C I, Hornillos R C, Diez L P

机构信息

Facultad de Ciencias Quimicas, Departamento de Quimica Analítica, Universidad Complutense de Madrid, Spain.

出版信息

J Chromatogr. 1988 Dec 23;458:217-23. doi: 10.1016/s0021-9673(00)90566-1.

DOI:10.1016/s0021-9673(00)90566-1

PMID:3235637

Abstract

A method is proposed for the determination of pterins in urine without clean-up by reversed-phase high-performance liquid chromatography on a C18 column previously conditioned with cetyltrimethylammonium bromide. Other endogenous compounds are retained in the column, preventing interference. Xanthopterin, neopterin, isoxanthopterin, monapterin, biopterin, 6-hydroxymethylpterin, pterin and lumazine can be determined. Retention and separation mechanisms are discussed.

摘要

提出了一种无需净化即可测定尿液中蝶呤的方法，该方法采用预先用十六烷基三甲基溴化铵进行预处理的C18柱，通过反相高效液相色谱法进行测定。其他内源性化合物保留在柱中，从而避免干扰。可测定黄蝶呤、新蝶呤、异黄蝶呤、单蝶呤、生物蝶呤、6-羟甲基蝶呤、蝶呤和鲁米诺嗪。讨论了保留和分离机制。