采用高效液相色谱法对生物体液中的蝶呤进行定量测定。

Quantitative determination of pterins in biological fluids by high-performance liquid chromatography.

作者信息

Stea B, Halpern R M, Halpern B C, Smith R A

出版信息

J Chromatogr. 1980 Feb 1;188(2):363-75. doi: 10.1016/s0021-9673(00)81259-5.

Abstract

During our continuing study of pteridine metabolism, the need arose for a more rapid and quantitative determination of pterins in biological fluids. By adopting and modifying previously developed techniques, we have obtained a rapid and sensitive method that allows the simultaneous determination of eight different pterins in human urine and blood. When examined over a 10-day period, the levels of pterins excreted by a normal individual averaged the following values expressed in picomoles per mg of creatinine: biopterin, 9104; neopterin, 6018; xanthopterin, 6561; pterin, 1136; isoxanthopterin, 636; pterin-6-carboxylate, 483; and 6-hydroxymethylpterin, 315. Moreover, 6-hydroxymethylpterin and pterin-6-carboxaldehyde were detected for the first time in the blood of normal individuals.

摘要

在我们对蝶啶代谢的持续研究中，需要一种更快速、定量地测定生物体液中蝶呤的方法。通过采用和改进先前开发的技术，我们获得了一种快速且灵敏的方法，可同时测定人尿液和血液中的八种不同蝶呤。在为期10天的检测期间，正常个体排泄的蝶呤水平平均如下，以每毫克肌酐中皮摩尔数表示：生物蝶呤，9104；新蝶呤，6018；黄蝶呤，6561；蝶呤，1136；异黄蝶呤，636；蝶呤-6-羧酸，483；以及6-羟甲基蝶呤，315。此外，在正常个体的血液中首次检测到6-羟甲基蝶呤和蝶呤-6-羧醛。