Taherdangkoo K, Kazemi Nezhad S R, Hajjari M R, Tahmasebi Birgani M
Bratisl Lek Listy. 2020;121(4):302-307. doi: 10.4149/BLL_2020_048.
Colorectal cancer (CRC), is the third most common cancer type. MicroRNAs and their roles in cancer progression have gained considerable attention in the scientific community. miR-485-3p has been identified to be abnormally expressed in different types of cancer, but its expression level, biological function, and underlying pathways are still unclear in CRC. Targeting Protein for Xenopus kinesin-like protein 2 (TPX2) is a nuclear protein which plays vital roles in cancer progression and mitotic spindle assembly. TPX2 is overexpressed in various malignancies and has been predicted as an indirect target of miR-485-3p. This study aims to investigate the miR-485-3p and TPX2 expression level, their potential correlation, and underlying molecules like P53 and P21 in forty-one pairs of colorectal cancer tissues compared to matched non-cancerous ones.
We used forty-one pairs of CRC fresh tissue samples and their adjacent normal ones for RNA extraction. After cDNA synthesis, the expression level of miR-485-3p, TPX2, P53 and P21 were determined by Real-time PCR.
The results revealed that miR-485-3p was significantly downregulated and TPX2 was highly upregulated in CRC tissues. Moreover, miR-485-3p was negatively correlated with TPX2 expression and positively correlated with P21 expression. We present miR-485-3p as a suppressor for colorectal cancer (Tab. 2, Fig. 8, Ref. 44).
结直肠癌（CRC）是第三大常见癌症类型。微小RNA及其在癌症进展中的作用在科学界已受到广泛关注。已确定miR-485-3p在不同类型癌症中异常表达，但在结直肠癌中其表达水平、生物学功能及潜在途径仍不清楚。靶向蛋白Xenopus驱动蛋白样蛋白2（TPX2）是一种核蛋白，在癌症进展和有丝分裂纺锤体组装中起重要作用。TPX2在各种恶性肿瘤中过表达，并被预测为miR-485-3p的间接靶点。本研究旨在调查41对结直肠癌组织与配对的癌旁组织中miR-485-3p和TPX2的表达水平、它们的潜在相关性以及P53和P21等潜在分子。
我们使用41对结直肠癌新鲜组织样本及其相邻正常组织进行RNA提取。cDNA合成后，通过实时定量PCR测定miR-485-3p、TPX2、P53和P21的表达水平。
结果显示，在结直肠癌组织中miR-485-3p显著下调，TPX2高度上调。此外，miR-485-3p与TPX2表达呈负相关，与P21表达呈正相关。我们提出miR-485-3p作为结直肠癌的一种抑制因子（表2，图8，参考文献44）。
