College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University, Daegu 41566, Republic of Korea.
College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University, Daegu 41566, Republic of Korea; Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State 39762, USA.
Poult Sci. 2020 May;99(5):2675-2683. doi: 10.1016/j.psj.2020.01.014. Epub 2020 Mar 18.
Enterococcus faecalis (E. faecalis) has rapidly acquired resistance to multiple antimicrobials, and the antimicrobial resistance of E. faecalis from broiler breeders has been implicated in its vertical transmission to their offspring. The objective of this study was to investigate the antimicrobial resistance and genetic diversity of commensal E. faecalis isolated from the broiler breeder farms. Among a total of 229 E. faecalis isolates from 9 broiler breeder farms, the highest resistance rate was observed in tetracycline (78.2%), followed by doxycycline (58.1%) and erythromycin (43.7%), and the prevalence of antimicrobial resistance showed significant differences among the 9 broiler breeder farms (P < 0.05). The tetM gene (77.1%) and ermB gene (85.0%) were detected at the highest levels in 179 TE-and 100 E-resistant isolates, respectively. Twenty-four high-level gentamicin-resistant isolates carried aac(6″)Ie-aph(2″)-la gene, and 9 high-level ciprofloxacin-resistant isolates showed point mutations in both gyrA and parC genes. All high-level gentamicin-resistant or high-level ciprofloxacin-resistant isolates showed one of the two different virulence gene patterns, ace-asa1-efaA-gelE complex or ace-efaA-gelE complex. These results indicate that constant epidemiological monitoring at the breeder level is required to prevent the pyramidal transmission of antimicrobial-resistant E. faecalis.
屎肠球菌（E. faecalis）已迅速获得对多种抗菌药物的耐药性，并且种鸡中屎肠球菌的抗菌药物耐药性已被认为与其垂直传播给后代有关。本研究旨在调查来自种鸡场的共生屎肠球菌的抗菌药物耐药性和遗传多样性。在来自 9 个种鸡场的总共 229 株屎肠球菌分离株中，四环素的耐药率最高（78.2%），其次是强力霉素（58.1%）和红霉素（43.7%），9 个种鸡场的抗菌药物耐药率存在显著差异（P < 0.05）。在 179 株耐四环素和 100 株耐红霉素的分离株中，检测到最高水平的 tetM 基因（77.1%）和 ermB 基因（85.0%）。24 株高水平庆大霉素耐药分离株携带 aac(6″)Ie-aph(2″)-la 基因，9 株高水平环丙沙星耐药分离株在 gyrA 和 parC 基因中均发生点突变。所有高水平庆大霉素耐药或高水平环丙沙星耐药分离株均表现出两种不同的毒力基因模式之一，即 ace-asa1-efaA-gelE 复合物或 ace-efaA-gelE 复合物。这些结果表明，需要在种鸡场进行持续的流行病学监测，以防止抗菌药物耐药屎肠球菌的金字塔式传播。
