完整细胞中血红蛋白的共振拉曼光谱：类风湿关节炎中红细胞摄取氧气的一种探测方法。
Resonance Raman spectroscopy of hemoglobin in intact cells: a probe of oxygen uptake by erythrocytes in rheumatoid arthritis.
作者信息
Hoey S, Brown D H, McConnell A A, Smith W E, Marabani M, Sturrock R D
机构信息
Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow.
出版信息
J Inorg Biochem. 1988 Nov;34(3):189-99. doi: 10.1016/0162-0134(88)85029-3.
Resonance Raman spectra from intact viable erythrocytes can be used to study oxygen uptake in solution. In addition to changes in the oxidation state marker (nu 4), other bands due to the porphyrin ring (nu 3) and vinyl modes indicate subtle changes at oxygen pressures close to where the T/R change occurs. A comparison of whole cell and lysate spectra indicates a partial denaturation of hemoglobin on lysis. A simple smear technique is used to measure spectra from rheumatoid and normal blood. Results indicate a faster but less complete uptake of oxygen in cells from patients with rheumatoid arthritis than is the case in normal cell populations.
完整活红细胞的共振拉曼光谱可用于研究溶液中的氧气摄取。除了氧化态标记物（ν4）的变化外，卟啉环（ν3）和乙烯基模式产生的其他谱带表明，在接近T/R变化发生的氧压下存在细微变化。全细胞光谱和裂解液光谱的比较表明，血红蛋白在裂解时会发生部分变性。一种简单的涂片技术用于测量类风湿性血液和正常血液的光谱。结果表明，类风湿性关节炎患者细胞对氧气的摄取比正常细胞群体更快，但不太完全。
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