Gautam Rekha, Oh Joo-Yeun, Marques Marisa B, Dluhy Richard A, Patel Rakesh P
Department of Pathology and Center for Free Radical Biology, University of Alabama at Birmingham, Birmingham, Alabama.
Department of Chemistry Pathology and Center for Free Radical Biology, University of Alabama at Birmingham, Birmingham, Alabama.
Lab Med. 2018 Oct 11;49(4):298-310. doi: 10.1093/labmed/lmy018.
The therapeutic efficacy and safety of stored red blood cells (RBCs) relies on minimal in-bag hemolysis. The accuracy of current methods of measuring hemolysis can suffer as a result of specimen collection and processing artefacts.
To test whether Raman spectroscopy could be used to assess hemolysis.
RBCs were stored for as long as 42 days. Raman spectra of RBCs were measured before and after washing, and hemolysis was measured in supernatant by visible spectroscopy.
Raman spectra indicated increased concentrations of oxyhemoglobin (oxyHb) and methemoglobin (metHb), and decreased membrane fluidity with storage age. Changes in oxyHb and metHb were associated with the intraerythrocytic and extracellular fractions, respectively. Hemolysis increased in a storage age-dependent manner. Changes in Raman bands reflective of oxyHb, metHb, and RBC membranes correlated with hemolysis; the most statistically significant change was an increased intensity of metHb and decreased membrane fluidity.
These data suggest that Raman spectroscopy may offer a new label-free modality to assess RBC hemolysis during cold storage.
储存红细胞（RBC）的治疗效果和安全性依赖于袋内最小程度的溶血。由于标本采集和处理假象，当前测量溶血方法的准确性可能受到影响。
测试拉曼光谱是否可用于评估溶血。
将红细胞储存长达42天。在洗涤前后测量红细胞的拉曼光谱，并通过可见光谱法测量上清液中的溶血情况。
拉曼光谱表明，随着储存时间的延长，氧合血红蛋白（oxyHb）和高铁血红蛋白（metHb）的浓度增加，膜流动性降低。oxyHb和metHb的变化分别与红细胞内和细胞外部分相关。溶血以储存时间依赖性方式增加。反映oxyHb、metHb和红细胞膜的拉曼谱带变化与溶血相关；统计学上最显著的变化是metHb强度增加和膜流动性降低。
这些数据表明，拉曼光谱可能提供一种新的无标记方法来评估冷藏期间红细胞的溶血情况。
