College of Pharmacy Touro University, Mare Island-Vallejo California, CA, 94592, USA.
Eur J Pharm Sci. 2020 Jul 1;150:105358. doi: 10.1016/j.ejps.2020.105358. Epub 2020 Apr 28.
Gene and nucleic acid delivery constitute a huge biological challenge and several attempts have been made by research laboratories to address this issue. Cationic polymers and cationic lipids (positively charged carriers) can be utilized for the transport of these biomolecules. Polyplexes (PPs) are interpolyelectrolyte complexes which are spontaneously formed through the electrostatic condensation between nucleic acid and a cationic polymer. PPs are capable of high-density payload condensation leading to cell internalization and subsequent protection from enzymatic degradation. Most cationic polymers can cross extracellular barriers, but it is more challenging to overcome intracellular barriers (efficient disassembly and endosomal escape). In this review, the use of PPs for gene and nucleic acid delivery is discussed.
基因和核酸递送构成了巨大的生物学挑战，研究实验室已经进行了多次尝试来解决这个问题。阳离子聚合物和阳离子脂质（带正电荷的载体）可用于这些生物分子的运输。聚电解质复合物（PPs）是通过核酸与阳离子聚合物之间的静电缩合而自发形成的。PPs 能够实现高密度的有效载荷缩合，从而导致细胞内化，并随后免受酶降解的影响。大多数阳离子聚合物可以跨越细胞外屏障，但克服细胞内屏障（有效解体和内体逃逸）更具挑战性。在这篇综述中，讨论了 PPs 在基因和核酸递送上的应用。
