SALUVET, Animal Health Department, Complutense University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain.
SALUVET, Animal Health Department, Complutense University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain; SALUVET-Innova S.L., Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain.
Int J Parasitol. 2020 May;50(5):377-388. doi: 10.1016/j.ijpara.2020.02.003. Epub 2020 Apr 28.
The biological variability among Neospora caninum isolates has been widely shown, however, the molecular basis that determines this diversity has not been thoroughly elucidated to date. The latest studies have focused on a limited number of isolates. Therefore, the goal of the present study was to compare the proteome of a larger number of N. caninum isolates with different origins and virulence. Label-free LC-MS/MS was used to investigate the tachyzoite proteomic differences among Nc-Bahia, Nc-Spain4H and Nc-Spain7, representing high virulence isolates and Nc-Ger6, Nc-Spain2H and Nc-Spain1H, representing low virulence isolates. Pairwise comparisons between all isolates and between high virulence and low virulence groups identified a subset of proteins with higher abundance in high virulence isolates. These proteins were involved in energy and redox metabolism, and DNA/RNA processing, which might determine the faster growth rates and parasite survival of the high virulence isolates. Highlighted proteins included a predicted member of the rhoptry kinase family ROP20 specific for N. caninum, Bradyzoite pseudokinase 1 and several dense granule proteins. DNA polymerase, which was more abundant in all high virulence isolates in all comparisons, might also be implicated in virulence. These results reveal insights into possible mechanisms involved in specific phenotypic traits and virulence in N. caninum, and the relevance of these candidate proteins for N. caninum virulence deserves further investigation.
目前，虽然已广泛证实刚地弓形虫分离株之间存在生物学变异性，但决定这种多样性的分子基础尚未得到充分阐明。最新的研究主要集中在少数几个分离株上。因此，本研究的目的是比较具有不同起源和毒力的大量刚地弓形虫分离株的蛋白质组。采用无标记 LC-MS/MS 技术研究了高毒力分离株 Nc-Bahia、Nc-Spain4H 和 Nc-Spain7 与低毒力分离株 Nc-Ger6、Nc-Spain2H 和 Nc-Spain1H 速殖子蛋白质组的差异。对所有分离株之间以及高毒力和低毒力组之间的两两比较鉴定出了一组在高毒力分离株中丰度更高的蛋白质。这些蛋白质参与能量和氧化还原代谢以及 DNA/RNA 加工，这可能决定了高毒力分离株更快的生长速度和寄生虫存活。突出的蛋白质包括刚地弓形虫特异性的预测 rhoptry 激酶家族成员 ROP20、原虫假激酶 1 和几个致密颗粒蛋白。在所有比较中，所有高毒力分离株中丰度更高的 DNA 聚合酶也可能与毒力有关。这些结果揭示了刚地弓形虫特定表型特征和毒力可能涉及的机制，并表明这些候选蛋白与刚地弓形虫毒力相关，值得进一步研究。
