Yao Mingze, Yang Qian, Lian Mengqiao, Su Pengfei, Cui Xiaogang, Ren Tinglin, Wang Xiedong, Wu Changxin, Zheng Yaowu
Institutes of Biomedical Sciences, Shanxi University, No. 92 Wucheng Road, Taiyuan 030006, China.
Institutes of Biomedical Sciences, Shanxi University, No. 92 Wucheng Road, Taiyuan 030006, China.
Stem Cell Res. 2020 May;45:101778. doi: 10.1016/j.scr.2020.101778. Epub 2020 Mar 19.
DIP2A mutation is associated with abnormal brain development and diseases including dyslexia, autism and Alzheimer's disease. However, the role and the involved mechanisms remain unknown. To study the biological function of DIP2A during mESCs neural differentiation in early neural development, we generated a Dip2a homozygous knockout 46C ESC cell line using CRISPR/Cas9 genome editing technology. The eighth exon of Dip2a gene was replaced with PGK-Puro-P2A-mCherry. This 46C-Dip2a KO cell line offers a useful resource to investigate the molecular mechanisms of DIP2A in the process of cell fate determination, as well as a potential source of building disease mouse model.
DIP2A突变与大脑发育异常以及包括诵读困难、自闭症和阿尔茨海默病在内的疾病相关。然而，其作用及相关机制仍不清楚。为了研究DIP2A在早期神经发育过程中mESCs神经分化期间的生物学功能，我们利用CRISPR/Cas9基因组编辑技术构建了Dip2a纯合敲除46C ESC细胞系。Dip2a基因的第八外显子被PGK-Puro-P2A-mCherry取代。这个46C-Dip2a KO细胞系为研究DIP2A在细胞命运决定过程中的分子机制提供了有用的资源，也是构建疾病小鼠模型的潜在来源。
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