The Third Hospital of Shanxi Medical University, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Taiyuan, China.
Department of Hepatobiliary Surgery, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Taiyuan, China.
Front Immunol. 2022 Jul 5;13:921900. doi: 10.3389/fimmu.2022.921900. eCollection 2022.
Hypersplenism (HS) is a concomitant symptom of liver or blood disease. Not only does the treatment of HS face challenges, but the transcriptome of individual cells is also unknown. Here, the transcriptional profiles of 43,037 cells from four HS tissues and one control tissue were generated by the single-cell RNA sequencing and nine major cell types, including T-cells, B-cells, NK cells, hematopoietic stem cells, neutrophil cells, mast cells, endothelial cells, erythrocytes, and dendritic cells were identified. Strikingly, the main features were the lack of B-cells in HS and the presence of B cells in HS with hepatocellular carcinoma (HS-HCC). In cell-cell interaction analysis, CD74-COPA and CD94-HLA-E in HS were found to be up-regulated. We further explored HS-specifically enriched genes (such as , , and ) and found that was highly expressed in HCC-HS, leading to immunosuppression. Taken together, this research provides new insights into the genetic characteristics of HS comprehensive single-cell transcriptome analysis.
脾功能亢进（HS）是肝脏或血液疾病的伴随症状。HS 的治疗不仅面临挑战，而且个体细胞的转录组也未知。在这里，通过单细胞 RNA 测序生成了来自四个 HS 组织和一个对照组织的 43037 个细胞的转录谱，并鉴定出了包括 T 细胞、B 细胞、NK 细胞、造血干细胞、中性粒细胞、肥大细胞、内皮细胞、红细胞和树突状细胞在内的 9 种主要细胞类型。值得注意的是，HS 的主要特征是缺乏 B 细胞，而在伴有肝细胞癌的 HS（HS-HCC）中存在 B 细胞。在细胞-细胞相互作用分析中，发现 HS 中的 CD74-COPA 和 CD94-HLA-E 上调。我们进一步探索了 HS 特异性富集的基因（如 、 和 ），发现 在 HCC-HS 中高表达，导致免疫抑制。总之，这项研究为 HS 的遗传特征提供了新的见解，即全面的单细胞转录组分析。
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