建立 CRISPR/Cas9 介导的 ANP32A 纯合敲除人胚胎干细胞系。
Establishment of a CRISPR/Cas9-mediated ANP32A homozygous knockout human embryonic stem cell line.
机构信息
CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Placenta Research Group, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
出版信息
Stem Cell Res. 2021 Apr;52:102234. doi: 10.1016/j.scr.2021.102234. Epub 2021 Feb 11.
ANP32A is a member of acidic leucine-rich nuclear phosphoprotein 32 family, which is involved in diverse biochemical processes, including chromatin modification and remodeling. Here, we established the CRISPR/Cas9-mediated ANP32A homozygous knockout human embryonic stem cell (ESC) line to investigate the roles of ANP32A in pluripotency maintenance and differentiation process of human ESCs. This cell line shows the normal karyotype and typical stem cell morphology, in accordance with high expression of pluripotent genes and the differentiation potential in vitro. Consequently, the ANP32A knockout cell line provides a promising approach for investigating the roles of ANP32A in human ESC cell fate decisions.
ANP32A 是酸性亮氨酸丰富核磷蛋白 32 家族的成员，参与多种生化过程，包括染色质修饰和重塑。在这里，我们建立了 CRISPR/Cas9 介导的 ANP32A 纯合敲除人胚胎干细胞 (ESC) 系，以研究 ANP32A 在人 ESC 多能性维持和分化过程中的作用。该细胞系显示正常核型和典型的干细胞形态，符合多能基因的高表达和体外分化潜能。因此，ANP32A 敲除细胞系为研究 ANP32A 在人 ESC 细胞命运决定中的作用提供了一种很有前途的方法。
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