Key Laboratory of Marine Environment and Ecology, Ministry of Education, Qingdao, Shandong Province, PR China; Ocean College of Hebei Agricultural University, Qinhuangdao, Hebei Province, PR China.
Key Laboratory of Marine Environment and Ecology, Ministry of Education, Qingdao, Shandong Province, PR China; College of Environmental Science and Engineering, Ocean University of China, Shandong Province, PR China.
Ecotoxicol Environ Saf. 2020 Jul 15;198:110687. doi: 10.1016/j.ecoenv.2020.110687. Epub 2020 Apr 30.
A marine diatom, Thalassiosira sp. OUC2, was isolated from natural seawater collected from Daya Bay, China. This diatom degraded 1.25-40 mg Lp-xylene within five days, at a removal efficiency exceeding 98%. Gas chromatography-mass spectrometer (GC-MS) analysis indicated that p-xylene was converted into 4-methylbenzyl alcohol, p-toluic acid, and p-cresol in the presence of strain OUC2. Meanwhile, proteomic analysis showed that, after exposure to p-xylene, several algal enzymes were significantly upregulated: including monooxygenase, alcohol dehydrogenase, benzaldehyde dehydrogenase, benzoate 1,2-dioxygenase, and catechol 2,3-dioxygenase. Moreover, ecotoxicological tests suggested that the intermediate metabolites were less toxic than the parent compound (p-xylene). Thalassiosira sp. OUC2 may thus be suitable for the remediation of p-xylene-contaminated marine environments.
一株海洋硅藻，Thalassiosira sp. OUC2，从中国大亚湾的天然海水中分离得到。该硅藻在五天内可降解 1.25-40mg/L 的对二甲苯，去除效率超过 98%。气相色谱-质谱联用仪（GC-MS）分析表明，在 OUC2 菌株的存在下，对二甲苯被转化为 4-甲基苄醇、对甲苯酸和对甲酚。同时，蛋白质组学分析表明，暴露于对二甲苯后，几种藻类酶的表达显著上调：包括单加氧酶、醇脱氢酶、苯甲醛脱氢酶、苯甲酸 1,2-双加氧酶和儿茶酚 2,3-双加氧酶。此外，生态毒理学试验表明，中间代谢物的毒性低于母体化合物（对二甲苯）。因此，Thalassiosira sp. OUC2 可能适用于修复受对二甲苯污染的海洋环境。
