Biochemistry Division, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Jl. Ganesha 10, Bandung 40132, Indonesia.
Bioresour Technol. 2012 Mar;108:240-4. doi: 10.1016/j.biortech.2011.12.082. Epub 2011 Dec 23.
To understand the potential of cultivating tropical marine diatom Thalassiosira sp. to produce biofuel, biodiesel product properties and growth characteristics of Thalassiosira sp. in three different media were investigated. After medium evaluation, significant Thalassiosira sp. cell growth was observed in both Walne and enriched seawater media, but not in plain seawater medium. The microalgae grew well in alkaline condition (pH range of 8.0-8.8). The average biomass density cultured in Walne and enriched seawater media on the 6th day was 4.36 and 2.50 g L(-1), respectively. Based on ESI-IT-MS spectra, the TAGs of algal oil were identified as POP, POO, and SOO, and the FAMEs as oleic acid methyl ester. The oil productivity of Thalassiosira sp. cultured in Walne and enriched seawater media were 150 and 290 μL L(-1) d(-1), respectively. The density and kinematic viscosity of Thalassiosira sp. biodiesel were 0.857 g mL(-1) and 1.151 mm(2) s(-1).
为了了解培养热带海洋硅藻塔玛斯藻（Thalassiosira sp.）生产生物燃料的潜力，研究了塔玛斯藻在三种不同培养基中的生物柴油产品特性和生长特性。在进行培养基评价后，发现塔玛斯藻在 Walne 和富化海水培养基中都有显著的细胞生长，但在普通海水培养基中没有。微藻在碱性条件（pH 值范围 8.0-8.8）下生长良好。第 6 天，在 Walne 和富化海水培养基中培养的平均生物量密度分别为 4.36 和 2.50 g L(-1)。根据 ESI-IT-MS 谱图，藻类油中的 TAG 被鉴定为 POP、POO 和 SOO，FAME 被鉴定为油酸甲酯。在 Walne 和富化海水培养基中培养的塔玛斯藻的产油率分别为 150 和 290 μL L(-1) d(-1)。塔玛斯藻生物柴油的密度和运动粘度分别为 0.857 g mL(-1)和 1.151 mm(2) s(-1)。
