Department of Zoology, Aligarh Muslim University, Aligarh, 202002, Uttar Pradesh, India.
Department of Zoology, Aligarh Muslim University, Aligarh, 202002, Uttar Pradesh, India.
Chemosphere. 2020 Sep;254:126875. doi: 10.1016/j.chemosphere.2020.126875. Epub 2020 Apr 24.
In the present scenario, insecticides/pesticides are used intensively to control the various insect pests. Indiscriminate use of these insecticides/pesticides affects the structure and function of the ecosystem. In this context, a thorough toxicological study of each insecticide/pesticide is a must to understand the hazardous effect of these chemicals on the target and non-target organisms. The present study was aimed to understand the hazardous effect of thiamethoxam against the Spodoptera litura. Different concentrations (20-80 μg/mL) of thiamethoxam were prepared, and fourth instar larvae of S. litura were allowed to feed for 12-72 h. We first examined the interaction of thiamethoxam with DNA. Next, treated and non-treated larvae were assessed for different biological parameters such as mortality, emergence, fecundity, fertility, longevities, and biochemical parameters. Our result showed that thiamethoxam directly interacts with the DNA and significantly influenced the different biological and biochemical parameters of exposed the organisms. We observed a significant change in stress enzymes such as SOD, CAT, and GST. A similar observation was also made with the oxidative marker for lipid damage, MDA and DNA damage, 8-OHdG, respectively. In conclusion, our results suggest that improper use of synthetic chemical insecticides influenced both biological and biochemical parameters through oxidative stress and probably damage the genetic material.
在当前的情况下，杀虫剂被广泛用于控制各种虫害。这些杀虫剂的滥用会影响生态系统的结构和功能。在这种情况下，对每种杀虫剂进行全面的毒理学研究是必要的，以了解这些化学物质对目标和非目标生物的危害。本研究旨在了解噻虫嗪对斜纹夜蛾的危害作用。用不同浓度（20-80μg/mL）的噻虫嗪处理四龄斜纹夜蛾幼虫，让其取食 12-72 小时。我们首先研究了噻虫嗪与 DNA 的相互作用。然后，评估了处理和未处理的幼虫的死亡率、羽化率、繁殖力、生育力、寿命以及生化参数等不同的生物学参数。我们的结果表明，噻虫嗪直接与 DNA 相互作用，显著影响了暴露于这些化学物质的生物体的不同生物学和生化参数。我们观察到应激酶如 SOD、CAT 和 GST 发生了显著变化。脂质损伤的氧化标记物 MDA 和 DNA 损伤标志物 8-OHdG 也观察到了类似的变化。总之，我们的研究结果表明，合成化学杀虫剂的不当使用会通过氧化应激影响生物和生化参数，并可能破坏遗传物质。
